AKT2 Protein
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- Target See all AKT2 Proteins
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Application
- Western Blotting (WB)
- Purification
- Recombinant protein corresponds to amino acids 1 to 479 of mature human AKT2, Akt isoform 2. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
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V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 1-123) protein (His-IF2DI Tag)
AKT2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 100-189) protein (GST tag)AKT2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) protein (GST tag,His tag)AKT2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (Active) protein (Myc-DYKDDDDK Tag)AKT2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, PI, STD Active
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 2-481) protein (His-SUMO Tag)AKT2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
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Application Note: AKT2 (phosphatase treated) is suitable as a control for polyclonal or monoclonal anti-AKT2 in immunological assays. Akt2 recombinant protein is dephosphorylated and in an inactive state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized.
Western Blot Dilution: 50 ng
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.17 mg/mL
- Buffer
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Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10 % glycerol
Stabilizer: 10 % (v/v) Glycerol
- Preservative
- Without preservative
- Storage
- -20 °C
- Storage Comment
- Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 12 months
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- Target
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- Alternative Name
- AKT2 (AKT2 Products)
- Synonyms
- AKT2 Protein, pkbb Protein, pkbbeta Protein, pkbss Protein, prkbb Protein, rac-beta Protein, v-akt2 Protein, 2410016A19Rik Protein, AW554154 Protein, PKB Protein, PKBbeta Protein, HIHGHH Protein, PKBB Protein, PKBBETA Protein, PRKBB Protein, RAC-BETA Protein, cb945 Protein, akt2-a Protein, AKT serine/threonine kinase 2 Protein, v-akt murine thymoma viral oncogene homolog 2 Protein, thymoma viral proto-oncogene 2 Protein, v-akt murine thymoma viral oncogene homolog 2 L homeolog Protein, AKT2 Protein, akt2 Protein, Akt2 Protein, akt2.L Protein
- Gene ID
- 208
- UniProt
- P31751
- Pathways
- PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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