NADH Dehydrogenase Protein (His tag)
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
1 image
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- Target
- NADH Dehydrogenase
- Protein Type
- Recombinant
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NADH Dehydrogenase protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Purified recombinant protein of Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22 kDa (NDUFB10), nuclear gene encoding mitochondrial protein, full length, with N-terminal HIS tag, expressed in E. coli, 50 μg (full length, N-term HIS tag) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
- Purification
- Purified
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- NADH Dehydrogenase
- Background
- Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 20.6 kDa
- NCBI Accession
- NP_004539
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