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SNCA Protein (full length)

Name: SNCA Protein (full length)
Brand: antibodies-online
SKU: ABIN5065829
Price: 629.2 USD
Availability: InStock

SNCA Origin: Human Host: Escherichia coli (E. coli) Recombinant >95% pure using SDS-PAGE analysis. SDS, WB, in vitro, in vivo Active

SDS-PAGE of ~14 kDa Active Human Recombinant Alpha Synuclein Protein Aggregate . (SNCA Protein (full length))

SDS

Toxicity results comparing Active Human Recombinant Alpha Synuclein Pre-formed Fibrils (Type 2) (Catalog No. (SNCA Protein (full length))

Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 µg/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 µg/ml (A-C). (SNCA Protein (full length))

IF/ICC

Primary rat hippocampal neurons show lewy body inclusion formation when treated with Type 1 Alpha Synuclein Protein Pre-formed Fibrils (ABIN5065829, ABIN5065830 and ABIN5564165) at 4 μg/mL (D-F), but not when treated with Type 2 Alpha Synuclein Protein Pre-formed Fibrils (ABIN5065829, ABIN5065830 and ABIN5564165) at 4 μg/mL (A-C). (SNCA Protein (full length))

ATTO633 fluorescently-labelled alpha synuclein PFFs (ABIN5065829, ABIN5065830 and ABIN5564165) were taken up, transported into the soma, and induced alpha synuclein aggregation in mouse neurocortical primary cells. (SNCA Protein (full length))

Confocal imaging shows NeuN+ (mature) primary cortical neurons filled with ATTO633 fluorescently-labelled alpha synuclein PFFs (ABIN5065829, ABIN5065830 and ABIN5564165). (SNCA Protein (full length))

Immunohistochemistry analysis of rat brain injected with Type 1 human alpha synuclein PFFs (ABIN5065829, ABIN5065830 and ABIN5564165). (SNCA Protein (full length))

IHC

TEM of Type 1 Alpha Synuclein Pre-formed Fibrils (PFFs) (ABIN5065829, ABIN5065830 and ABIN5564165) (SNCA Protein (full length))

Type 1 alpha synuclein Pre-formed Fibrils (ABIN5065829, ABIN5065830 and ABIN5564165) seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers (ABIN5065829, ABIN5065830 and ABIN5564165). (SNCA Protein (full length))

10 images

Catalog No. ABIN5065829

Datasheet as PDF

Target See all SNCA Proteins

SNCA (Synuclein, alpha (SNCA))

Protein Type

Recombinant

Biological Activity

Active

Protein Characteristics

full length
Origin
25

6

4

2

1

1

1

1

1

1

1

1

1

1
Human
Source
23

13

3

2

2
Escherichia coli (E. coli)

Application

SDS-PAGE (SDS), Western Blotting (WB), In vitro Assay (in vitro), In vivo Studies (in vivo)

Sequence

MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Specificity

~14.46 kDa

Purification

Ion-exchange Purified

Purity

>95% pure using SDS-PAGE analysis.

Biological Activity Comment

Endogenous alpha-synuclein phosphorylation. 100 μM alpha synuclein protein monomer (SPR-321) seeded with 10 nM alpha synuclein protein PFF (SPR-322) in 25 μM Thioflavin T (PBS pH 7.4, 100 μl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.

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Application Notes

Optimal working dilution should be determined by the investigator.

Comment

Certified >95% pure using SDS-PAGE analysis.

Restrictions

For Research Use only
Concentration

Lot specific

Buffer

PBS

Storage

-80 °C
Target

SNCA (Synuclein, alpha (SNCA))

Alternative Name

Alpha Synuclein (SNCA Products)

Synonyms

snca Protein, MGC64356 Protein, LOC619283 Protein, NACP Protein, alphaSYN Protein, PARK1 Protein, PARK4 Protein, PD1 Protein, synuclein alpha L homeolog Protein, alpha-synuclein Protein, synuclein alpha Protein, synuclein, alpha Protein, snca.L Protein, LOC619283 Protein, SNCA Protein, Snca Protein

Background

Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions, inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).

Molecular Weight

14.46 kDa

Gene ID

6622

NCBI Accession

NP_000336

UniProt

P37840

Pathways

Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity