MLL2-SET (AA 5355-5537) protein (GST tag)
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- Target
- MLL2-SET
- Protein Type
- Recombinant
- Protein Characteristics
- AA 5355-5537
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- GST tag
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- The peptide corresponding to amino acids 5355 - 5537 of the MLL2 protein (accession number NP_003473.3) was expressed in E. coli. The peptide contains the SET domain of MLL2 that is responsible for methyltransferase activity. Recombinant MLL2-SET contains an N-terminal GST tag with a molecular weight of 47.6 kDa.
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- Application Notes
- Recombinant MLL2-SET is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- MLL2-SET
- Background
- Myeloid/lymphoid or mixed-lineage leukemia 2 (MLL2) is a Trithorax-group protein that function collectively to promote gene expression. MLL2 is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. MLL2 is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the β-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome.
- Molecular Weight
- 47.6 kDa
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