SMAD4 Protein (AA 1-552) (His tag)
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- Target See all SMAD4 Proteins
- SMAD4 (SMAD Family Member 4 (SMAD4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-552
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SMAD4 protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)
- Purpose
- Recombinant Human SMAD4 Protein is produced by our E. coli expression system and the target gene encoding Met1-Asp552 is expressed with a 6His tag at the C-terminus.
- Sequence
- MDNMSITNTP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK KDELDSLITA ITTNGAHPSK CVTIQRTLDG RLQVAGRKGF PHVIYARLWR WPDLHKNELK HVKYCQYAFD LKCDSVCVNP YHYERVVSPG IDLSGLTLQS NAPSSMMVKD EYVHDFEGQP SLSTEGHSIQ TIQHPPSNRA STETYSTPAL LAPSESNATS TANFPNIPVA STSQPASILG GSHSEGLLQI ASGPQPGQQQ NGFTGQPATY HHNSTTTWTG SRTAPYTPNL PHHQNGHLQH HPPMPPHPGH YWPVHNELAF QPPISNHPAP EYWCSIAYFE MDVQVGETFK VPSSCPIVTV DGYVDPSGGD RFCLGQLSNV HRTEAIERAR LHIGKGVQLE CKGEGDVWVR CLSDHAVFVQ SYYLDREAGR APGDAVHKIY PSAYIKVFDL RQCHRQMQQQ AATAQAAAAA QAAAVAGNIP GPGSVGGIAP AISLSAAAGI GVDDLRRLCI LRMSFVKGWG PDYPRQSIKE TPCWIEIHLH RALQLLDEVL HTMPIADPQP LDLEHHHHHH
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
- Top Product
- Discover our top product SMAD4 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM Tris-HCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- SMAD4 (SMAD Family Member 4 (SMAD4))
- Alternative Name
- SMAD4 (SMAD4 Products)
- Synonyms
- smad4 Protein, DPC4 Protein, JIP Protein, MADH4 Protein, MYHRS Protein, AW743858 Protein, D18Wsu70e Protein, Madh4 Protein, XSmad4alpha Protein, Xsmad4 Protein, dpc4 Protein, jip Protein, madh4 Protein, xsmad4a Protein, SMAD family member 4 Protein, Smad4 protein Protein, SMAD family member 4, gene 1 L homeolog Protein, SMAD4 Protein, smad4 Protein, Smad4 Protein, smad4.1.L Protein
- Gene ID
- 4089
- UniProt
- Q13485
- Pathways
- Cell Division Cycle, Chromatin Binding, Autophagy
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