LacI (AA 1-360) protein (His tag)
Origin: E. coli
Host: Escherichia coli (E. coli)
Recombinant
Greater than 95 % as determined by reducing SDS-PAGE.
ELISA, MS, SDS, WB
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- Target
- LacI
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-360
- Origin
- E. coli
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- ELISA, Mass Spectrometry (MS), SDS-PAGE (SDS), Western Blotting (WB)
- Purpose
- Recombinant E. coli LACI Protein is produced by our E. coli expression system and the target gene encoding Met1-Gln360 is expressed with a 6His tag at the C-terminus.
- Sequence
- MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ HHHHHH
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as a 0.2 μM filtered solution of 20 mM Tris, 300 mM NaCl, 5 mM DTT, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- LacI
- Synonyms
- ECK0342 Protein, JW0336 Protein, lactose-inducible lac operon transcriptional repressor Protein, lacI Protein
- Gene ID
- 945007
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