IgE Protein (Biotin)

Catalog No. ABIN457505

Product Details

Target: IgE

Protein Type: Recombinant

Origin: Human

Source: Human

Purification tag / Conjugate: This IgE protein is labelled with Biotin.

Application: Western Blotting (WB), Isotype Control (IsoC), ELISA, Functional Studies (Func), Flow Cytometry (FACS)

Specificity: Binds with Fc effector domain to human and non human primate IgE receptors, no cross-reactivity with rodent or canine IgE receptors, specific antigen unknown

Sensitivity: Reactivity > 99 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone SUS-11)

Characteristics: IGHE, chromosome 14q32.33 Source: Human B cell hybridoma from healthy donors (CD40 cell culture system)

Purification: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - mouse anti-human IgE mAb (BSW17)

Purity: > 95 %, tested by analytical Gel Permeation Chromatography

Application Details

Application Notes: For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation (Cat. No. 0911-1-005-B) is recommended

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, pH=7.2, 0.1 % NaN3

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C

References

Kim, Jo, Lim, Kim, Eun, Oh, Kim, Cho, Kim: "Enhanced Type 2 Immune Reactions by Increased IL-22/IL-22Ra1 Signaling in Chronic Rhinosinusitis With Nasal Polyps." in: Allergy, asthma & immunology research, Vol. 12, Issue 6, pp. 980-993, (2020) (PubMed).

Target Details

Target: IgE

Abstract: IgE Products

Synonyms: IGH2 Protein, IgE Protein, Gm900 Protein, immunoglobulin heavy chain (epsilon polypeptide) Protein, immunoglobulin heavy constant epsilon Protein, Immunoglobulin heavy constant epsilon Protein, Ighe Protein, IGHE Protein

Background: Recombinant human IgE to be used as reference for IgE concentration measurements in biological samples (ELISA, FACS). IgE (SUS-11) recognizes the human high affinity IgE receptor (FceRIa), the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with either anti-human IgE mAbs mAb LE27 (Cat. No. 0908-1-100 or a specific recombinant cross-linking agent.