HMGB3 Protein (AA 2-200) (His tag)
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- Target See all HMGB3 Proteins
- HMGB3 (High Mobility Group Box 3 (HMGB3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-200
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This HMGB3 protein is labelled with His tag.
- Purpose
- Recombinant Human High Mobility Group Protein B3/HMGB3 (C-6His)
- Sequence
- MAKGDPKKPK GKMSAYAFFV QTCREEHKKK NPEVPVNFAE FSKKCSERWK TMSGKEKSKF DEMAKADKVR YDREMKDYGP AKGGKKKKDP NAPKRPPSGF FLFCSEFRPK IKSTNPGISI GDVAKKLGEM WNNLNDSEKQ PYITKAAKLK EKYEKDVADY KSKGKFDGAK GPAKVARKKV EEEDEEEEEE EEEEEEEEDE VDHHHHHH
- Characteristics
- Recombinant Human High Mobility Group Protein B3/HMGB3 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Ala2-Glu200) of Human HMGB3 fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product HMGB3 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- HMGB3 (High Mobility Group Box 3 (HMGB3))
- Alternative Name
- HMGB3 (HMGB3 Products)
- Synonyms
- HMG-2a Protein, HMG-4 Protein, HMG2A Protein, HMG4 Protein, Hmg2a Protein, Hmg4 Protein, RGD1564407 Protein, hmgb3 Protein, MGC54022 Protein, Xhmgb3 Protein, MGC88931 Protein, fa19b06 Protein, fj43d02 Protein, wu:fa19b06 Protein, wu:fj43d02 Protein, zgc:112073 Protein, HMGB3 Protein, NFD03 Protein, NFD3 Protein, NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR Protein, high mobility group B3 Protein, HMG-1 Protein, HMG2a Protein, HMGB1 Protein, high mobility group box 3 Protein, high mobility group box 3b Protein, high mobility group box 3 S homeolog Protein, high mobility group protein Protein, high mobility group B3 Protein, High mobility group protein B3 Protein, HMGB3 Protein, Hmgb3 Protein, hmgb3 Protein, hmgb3b Protein, hmgb3.S Protein
- Sub Type
- Fusionprotein
- Background
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High Mobility Group Protein B3 (HMGB3) belongs to the HMGB family. Members of the HMG box subfamily are thought to be have an important role in DNA replication, nucleosome assembly and transcription. HMGB3 binds preferentiallly single-stranded DNA and unwinds double stranded DNA. HMGB3 consists of 200 amino acids and is localized to the cell nucleus. It contains two HMG box DNA-binding domain. HMGB3 binds preferentially single-stranded DNA and unwinds double stranded DNA.
Alternative Names: High Mobility Group Protein B3, High Mobility Group Protein 2a, HMG-2a, High Mobility Group Protein 4, HMG-4, HMGB3, HMG2A, HMG4 - Molecular Weight
- 24 kDa
- UniProt
- O15347
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