Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Protein L Protein (His tag)

Origin: Peptostreptococcus magnus Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE.
Catalog No. ABIN2181671
  • Target See all Protein L Proteins
    Protein L
    Protein Type
    Recombinant
    Origin
    • 4
    • 1
    Peptostreptococcus magnus
    Source
    • 3
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This Protein L protein is labelled with His tag.
    Brand
    MABSol®
    Characteristics
    Recombinant Protein L Protein is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 36-38 kDa polypeptide .Protein L Protein can bind to VL-Kappa.
    Purity
    >95 % as determined by SDS-PAGE.
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
    Buffer
    100 mM NaH2PO4, pH 8.5
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
  • Martinović, Andjelković, Klobučar, Černigoj, Vidič, Lučić, Pavelić, Josić: "Affinity chromatography on monolithic supports for simultaneous and high-throughput isolation of immunoglobulins from human serum." in: Electrophoresis, Vol. 38, Issue 22-23, pp. 2909-2913, (2017) (PubMed).

  • Target
    Protein L
    Abstract
    Protein L Products
    Background
    Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).
    Gene ID
    17
You are here:
Support