CNGA2 Protein (AA 1-664) (Strep Tag)
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- Target See all CNGA2 Proteins
- CNGA2 (Cyclic Nucleotide Gated Channel alpha 2 (CNGA2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-664
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This CNGA2 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MMTEKSNGVK SSPANNHNHH PPPSIKANGK DDHRAGSRPQ SVAADDDTSS ELQRLAEMDT PRRGRGGFRR IVRLVGIIRD WANKNFREEE PRPDSFLERF RGPELQTVTT HQGDGKGDKD GEGKGTKKKF ELFVLDPAGD WYYRWLFVIA MPVLYNWCLL VARACFSDLQ RNYFVVWLVL DYFSDTVYIA DLIIRLRTGF LEQGLLVKDP KKLRDNYIHT LQFKLDVASI IPTDLIYFAV GIHSPEVRFN RLLHFARMFE FFDRTETRTS YPNIFRISNL VLYILVIIHW NACIYYAISK SIGFGVDTWV YPNITDPEYG YLAREYIYCL YWSTLTLTTI GETPPPVKDE EYLFVIFDFL IGVLIFATIV GNVGSMISNM NATRAEFQAK IDAVKHYMQF RKVSKDMEAK VIKWFDYLWT NKKTVDEREV LKNLPAKLRA EIAINVHLST LKKVRIFQDC EAGLLVELVL KLRPQVFSPG DYICRKGDIG KEMYIIKEGK LAVVADDGVT QYALLSAGSC FGEISILNIK GSKMGNRRTA NIRSLGYSDL FCLSKDDLME AVTEYPDAKK VLEERGREIL MKEGLLDENE VAASMEVDVQ EKLEQLETNM ETLYTRFARL LAEYTGAQQK LKQRITVLET KMKQNHEDDY LSDGINTPEP AVAE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- CNGA2 (Cyclic Nucleotide Gated Channel alpha 2 (CNGA2))
- Alternative Name
- Cnga2 (CNGA2 Products)
- Synonyms
- Cncg4 Protein, OLFCH Protein, CNCA Protein, CNCA1 Protein, CNG2 Protein, OCNC1 Protein, OCNCALPHA Protein, OCNCa Protein, CNCG2 Protein, CNGA2 Protein, Cnca Protein, cyclic nucleotide gated channel alpha 2 Protein, Cnga2 Protein, CNGA2 Protein, cnga2 Protein
- Background
- Cyclic nucleotide-gated olfactory channel (Cyclic nucleotide-gated cation channel 2) (Cyclic nucleotide-gated channel alpha-2) (CNG channel alpha-2) (CNG-2) (CNG2),FUNCTION: Odorant signal transduction is probably mediated by a G-protein coupled cascade using cAMP as second messenger. The olfactory channel can be shown to be activated by cyclic nucleotides which leads to a depolarization of olfactory sensory neurons.
- Molecular Weight
- 76.2 kDa
- UniProt
- Q62398
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