LASS5 Protein (AA 1-414) (Strep Tag)
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- Target See all LASS5 Proteins
- LASS5 (LAG1 Homolog, Ceramide Synthase 5 (LASS5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-414
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This LASS5 protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
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MATAAAETLG LLWGWLWSES FWLPQNVSWA DLEGPGDGYG YPRAQHVLSV FPLAVCIFSV RMLFERFIAK PCALRVGIKD SPVNKVEPND TLEKVFVSVT KYPDEKRLKG LSKQLDWSVR KIQCWFRHRR NQDKPPTLTK FCESMWRFTY YLCIFCYGIR FLWSMPWFWD TRQCWYNYPY QPLSRELYYY YITQLAFYWS LMFSQFIDVK RKDFLMMFIH HMIGIMLTTF SYVNNMVRVG ALIFCLHDFA DPLLEAAKMA NYARRERLCT TLFVIFGAAF IVSRLAIFPL WILNTTLFES WEIIGPYPSW WLFNALLLIL QVLHAIWSYL IVQTASKALS RGKVSKDDRS DVESSSEEED ETTHKNNLSG SSSSNGANCM NGYMGGSHLA EEQGTCKATG NLHFRASPHL HSCD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product LASS5 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- LASS5 (LAG1 Homolog, Ceramide Synthase 5 (LASS5))
- Alternative Name
- Cers5 (LASS5 Products)
- Synonyms
- lass5 Protein, trh4 Protein, LASS5 Protein, Trh4 Protein, 2310081H14Rik Protein, AU045339 Protein, AW544927 Protein, Lass5 Protein, ceramide synthase 5 Protein, ceramide synthase 5 S homeolog Protein, CERS5 Protein, cers5 Protein, cers5.S Protein, Cers5 Protein
- Background
- Ceramide synthase 5 (CerS5) (LAG1 longevity assurance homolog 5) (Sphingoid base N-palmitoyltransferase CERS5) (EC 2.3.1.291) (Sphingosine N-acyltransferase CERS5) (EC 2.3.1.24) (Translocating chain-associating membrane protein homolog 4) (TRAM homolog 4),FUNCTION: Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward palmitoyl-CoA (hexadecanoyl-CoA, C16:0-CoA) (PubMed:12912983, PubMed:15823095, PubMed:16100120, PubMed:17977534, PubMed:17609214, PubMed:26853464). Can use other acyl donors, but with less efficiency (PubMed:15823095). N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively (PubMed:12912983, PubMed:15823095, PubMed:15772421, PubMed:17977534). Plays a role in de novo ceramide synthesis and surfactant homeostasis in pulmonary epithelia (PubMed:15772421). {ECO:0000269|PubMed:12912983, ECO:0000269|PubMed:15772421, ECO:0000269|PubMed:15823095, ECO:0000269|PubMed:16100120, ECO:0000269|PubMed:17609214, ECO:0000269|PubMed:17977534, ECO:0000269|PubMed:26853464}.
- Molecular Weight
- 48.2 kDa
- UniProt
- Q9D6K9
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