SLC5A6 Protein (AA 1-634) (Strep Tag)
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- Target See all SLC5A6 products
- SLC5A6 (Solute Carrier Family 5 (Iodide Transporter), Member 6 (SLC5A6))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-634
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This SLC5A6 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MSVASTAAPF HTTSGSSGAI STFSVVDYVV FGLLLVLSLV IGLYHACRGW GHHTVGELLM ADRKMGCLPV ALSLLATFQS AVAILGAPAE IFRFGTQYWF LGCSYFLGLL IPAHIFIPVF YRLHLTSAYE YLELRFNKAV RICGTVTFIF QMVIYMGVAL YAPSLALNAV TGFDLWLSVL ALGIVCNIYT ALGGLKAVIW TDVFQTLVMF LGQLVVIIVG AARVGGLGHV WNVTSQHGLI SGINLDPDPF VRHTFWTLAF GGVFMMLSLY GVNQAQVQRY LSSHSERAAV LSCYAVFPCQ QVALCMSCLI GLVMFAYYNM YSMSPELKQA APDQLVLYFV MDLLKDMPGL PGLFVACLFS GSLSTISSAF NSLATVTMED LIQPWFPQLT ETRAIMLSRG LAFAYGLVCL GMAYISSHLG SVLQAALSIF GMVGGPLLGL FCLGLFFPCA NPLGAIVGLL TGLTMAFWIG IGSIVSRMSS AVAPPPLNGS SSFLPANVTV AAVTTVMPST LSKPTGLQHF YSLSYLWYSA HNSTTVIVVG LIVSLLTGGM RGRSLNPGTI YPVLPKLLAL LPLSCQKRLC WRSHSQDIPV IPNLFPEKMR NGVLQDSTDK ERMAEDGLVH QPCSPTYVVQ ETSL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- SLC5A6 (Solute Carrier Family 5 (Iodide Transporter), Member 6 (SLC5A6))
- Alternative Name
- Slc5a6 (SLC5A6 Products)
- Synonyms
- SLC5A6 Protein, Slc5a6 Protein, E430023I20 Protein, SMVT Protein, solute carrier family 5 member 8 Protein, solute carrier family 5 member 6 Protein, solute carrier family 5 (sodium-dependent vitamin transporter), member 6 Protein, SLC5A8 Protein, SLC5A6 Protein, Slc5a6 Protein
- Background
- Sodium-dependent multivitamin transporter (Na(+)-dependent multivitamin transporter) (Solute carrier family 5 member 6),FUNCTION: Sodium-dependent multivitamin transporter that mediates the electrogenic transport of pantothenate, biotin, lipoate and iodide (PubMed:23104561). Functions as a Na(+)-coupled substrate symporter where the stoichiometry of Na(+):substrate is 2:1, creating an electrochemical Na(+) gradient used as driving force for substrate uptake (By similarity). Required for biotin and pantothenate uptake in the intestine across the brush border membrane (PubMed:23104561). Plays a role in the maintenance of intestinal mucosa integrity, by providing the gut mucosa with biotin (PubMed:27492331). Contributes to the luminal uptake of biotin and pantothenate into the brain across the blood-brain barrier (By similarity). {ECO:0000250|UniProtKB:Q9Y289, ECO:0000269|PubMed:23104561, ECO:0000269|PubMed:27492331}.
- Molecular Weight
- 68.5 kDa
- UniProt
- Q5U4D8
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