ALG11 Protein (AA 1-492) (Strep Tag)
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- Target See all ALG11 Proteins
- ALG11 (Asparagine-Linked Glycosylation 11, alpha-1,2-Mannosyltransferase Homolog (Yeast) (ALG11))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-492
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Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This ALG11 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MAADTGSWCV YAVLRFFYSL FFPGLMICGV LCVYLVIGLW VIRWHLQRKK KSVSTSKNGK EQTVVAFFHP YCNAGGGGER VLWCALRALQ KKYPEAVYVV YTGDINVSGQ QILDGAFRRF NIKLAHPVQF VFLRKRYLVE DSRYPHFTLL GQSLGSILLG WEALMQRVPD VYIDSMGYAF TLPLFKYVGG CRVGSYVHYP TISTDMLSVV KNQNPGFNNA AFISRNALLS KAKLIYYYLF AFVYGLVGSC SDIVMVNSSW TLNHILSLWK VGHCTNIVYP PCDVQTFLDI PLHEKKVTPG HLLVSIGQFR PEKNHALQIK AFAKLLNEKA AELGHSLKLV LIGGCRNKDD EFRVNQLRSL SENLGVQENV EFKINISFDE LKNYLSEATI GLHTMWNEHF GIGVVECMAA GTVILAHNSG GPKLDIVIPH EGQITGFLAE SEEGYADSMA HILSLSAEER LQIRKNARAS ISRFSDQEFE VAFLCSMEKL LT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ALG11 (Asparagine-Linked Glycosylation 11, alpha-1,2-Mannosyltransferase Homolog (Yeast) (ALG11))
- Alternative Name
- Alg11 (ALG11 Products)
- Synonyms
- UTP14C Protein, CDG1P Protein, GT8 Protein, RGD1564725 Protein, AI849156 Protein, AW492253 Protein, B230397C21 Protein, si:dkey-1h24.5 Protein, wu:fj04e04 Protein, asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase Protein, ALG11, alpha-1,2-mannosyltransferase Protein, ALG11, alpha-1,2-mannosyltransferase L homeolog Protein, asparagine-linked glycosylation 11 (alpha-1,2-mannosyltransferase) Protein, alg11 Protein, ALG11 Protein, alg11.L Protein, Alg11 Protein
- Background
- GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (EC 2.4.1.131) (Asparagine-linked glycosylation protein 11 homolog) (Glycolipid 2-alpha-mannosyltransferase),FUNCTION: Mannosyltransferase involved in the last steps of the synthesis of Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum. Catalyzes the addition of the 4th and 5th mannose residues to the dolichol-linked oligosaccharide chain (By similarity). {ECO:0000250}.
- Molecular Weight
- 55.3 kDa
- UniProt
- Q3TZM9
- Pathways
- SARS-CoV-2 Protein Interactome
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