ROR1 Protein (AA 30-403) (Fc Tag,AVI tag,Biotin)
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- Target See all ROR1 Proteins
- ROR1 (Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 30-403
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ROR1 protein is labelled with Fc Tag,AVI tag,Biotin.
- Sequence
- AA 30-403
- Specificity
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Characteristics
- This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 70.4 kDa. As a result of Glycosylation, the protein migrates as 79-104 kDa under reducing (R) condition, and 158-208 kDa under non-reducing (NR) condition (SDS-PAGE).
- Purity
- >95 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product ROR1 Protein
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- Comment
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH 7.5
- Handling Advice
- Please avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
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- Target
- ROR1 (Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1))
- Alternative Name
- ROR1 (ROR1 Products)
- Synonyms
- NTRKR1 Protein, dJ537F10.1 Protein, 2810404D04Rik Protein, Ntrkr1 Protein, mRor1 Protein, RGD1559469 Protein, ROR1 Protein, fi34e02 Protein, si:dkey-209n16.1 Protein, wu:fi34e02 Protein, receptor tyrosine kinase like orphan receptor 1 Protein, receptor tyrosine kinase-like orphan receptor 1 Protein, ROR1 Protein, Ror1 Protein, ror1 Protein
- Background
- Tyrosine-protein kinase transmembrane receptor ROR1 is also known as Neurotrophic tyrosine kinase, receptor-related 1 (NTRKR1), which belongs to the protein kinase superfamily or tyr protein kinase family or ROR subfamily. ROR1 contains 1 FZ (frizzled) domain, 1 Ig-like C2-type (immunoglobulin-like) domain, 1 kringle domain, 1 protein kinase domain. ROR1 is expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. ROR1 could interact with casein kinase 1 epsilon (CK1ε) to activate phosphoinositide 3-kinase-mediated AKT phosphorylation and cAMP-response-element-binding protein (CREB), which was associated with enhanced tumor-cell growth.
- Molecular Weight
- 70.1 kDa
- Pathways
- RTK Signaling, WNT Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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