HEY1 Protein (AA 1-299) (Strep Tag)
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- Target See all HEY1 Proteins
- HEY1 (Ha-Ry/enhancer-of-Split Related with YRPW Motif 1 (HEY1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-299
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Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This HEY1 protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
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MKRAHPDYSS SDSELDETIE VEKESADENG NLSSALCSMS PTTSSQVLAR KRRRGIIEKR RRDRINNSLS ELRRLVPSAF EKQGSAKLEK AEILQMTVDH LKMLHTAGGK GYFDAHALAM DYRSLGFREC LAEVARYLSI IEGLDASDPL LVRLVSHLNN YASQREAASG AHGGLGHIPW GSAFGHHPHI AHPLLLPQNG HGNAGTAASP TEPHHQGRLA SAHPEAPALR APPSGGLGPV LPVVTSASKL SPPLLSSVAS LSAFPFSFSS FHLLSPSTPT QAANLGKPYR PWGTEIGAF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product HEY1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- HEY1 (Ha-Ry/enhancer-of-Split Related with YRPW Motif 1 (HEY1))
- Alternative Name
- Hey1 (HEY1 Products)
- Synonyms
- BHLHb31 Protein, CHF2 Protein, HERP2 Protein, HESR1 Protein, HRT-1 Protein, OAF1 Protein, AI316788 Protein, AI414254 Protein, HRT1 Protein, Herp2 Protein, Hesr1 Protein, bHLHb31 Protein, hesr-1 Protein, id:ibd1292 Protein, zgc:110572 Protein, bc8 Protein, chf2 Protein, herp2 Protein, hesr1 Protein, hrt1 Protein, oaf1 Protein, xbc8 Protein, hes related family bHLH transcription factor with YRPW motif 1 Protein, hairy/enhancer-of-split related with YRPW motif 1 Protein, hes-related family bHLH transcription factor with YRPW motif 1 Protein, hes related family bHLH transcription factor with YRPW motif 1 S homeolog Protein, HEY1 Protein, Hey1 Protein, hey1 Protein, hey1.S Protein
- Background
- Hairy/enhancer-of-split related with YRPW motif protein 1 (Hairy and enhancer of split-related protein 1) (HESR-1) (Hairy-related transcription factor 1) (HRT-1) (mHRT1),FUNCTION: Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Downstream effector of Notch signaling required for cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY2 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3. {ECO:0000269|PubMed:11095750, ECO:0000269|PubMed:11486045, ECO:0000269|PubMed:12947105, ECO:0000269|PubMed:15107403, ECO:0000269|PubMed:15680351, ECO:0000269|PubMed:16199874, ECO:0000269|PubMed:17259303, ECO:0000269|PubMed:17303760}.
- Molecular Weight
- 32.1 kDa
- UniProt
- Q9WV93
- Pathways
- Tube Formation
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