CCDC39 Protein (AA 1-937) (Strep Tag)
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- Target See all CCDC39 products
- CCDC39 (Coiled-Coil Domain Containing 39 (CCDC39))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-937
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This CCDC39 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MCSEFLSELH WEDGFAIPVA NQENKILEDQ LAKLREEKSN LQDQLHDYEE RINSMTSHLK NVNQEFLFTQ SLYKARESEI ESEEHFKAIA ERELGRVKNE TQLLEKEMAI IRERKSQMEN NIFKTTQKLD DLKCQMNWDQ QALEAWLEES AHKDSDSLTL QKYSQQDDNK IRALTLQLEK LTMEYNEKRK LLDSELTETL SAQLELDKAA QDFRKIHLER QELIQQWENT IEQMQRRDQE IDNCALALSR IKQEAREKEG VVKEKIKFLE NEVENNIEYE RKISVAERKV SKCRMDYQRH EGNRSQLKDE LDTLKTTLNR TSSDLQALRK NISKVKKDIF DETLRLQKLK HHNEVVKHKL KMITEKTLSI EEKATNMEDM LKEEEKGLKE VEVQLGIVKD VLFKKVQELQ NEIAKEKALV SEIEGTRSSL KHLNKQLHKL DFETLKQQEI MYSQDFYIQQ VERRMSRLKG EINSEEKQAL EAKILELKKT MDEKKSTLSL LESQIKKLHN DLYFIKKSNG KNNDEKESLM NKISELNLFI DRSEKELSKA KAVKEDMMIE DNLLKLQVKR ARELLYSKAE EVLSLEKRKQ QLGKDMEERA EEIKVHKAML TSQIRCVEQQ RKTMSSEFHE RLSKIDKLKN RYEILTVVML PPEGEEEKTQ SYYVIKAAQE KEELQREGDS LDAKINKAEK EIYALQNTLQ VLNSCNSNYK QSFKKVTPSS DEYALKIQLE EQKRTADERY RCKQRQIREL QEDIQSMENT FEVIGHLANN AKEKLTEKQT LAFQLRKETE EQKPKLQRIT KQCGRLRREI RILKQTDNET LEEQDIQLRE IIQFHKDIDQ MLVNAMENAE IHAIFKTYFE QNGLELPTAR GPSSRSSSQS SSLSSFRSLE DVTLQSPPTA KVIQLRFPEP PPATNDSSRS ASSGSNSNIP KEKKLSK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- CCDC39 (Coiled-Coil Domain Containing 39 (CCDC39))
- Alternative Name
- Ccdc39 (CCDC39 Products)
- Synonyms
- 4921507O14Rik Protein, AI447426 Protein, AI844750 Protein, D3Ertd789e Protein, b2b1304Clo Protein, b2b1735Clo Protein, RGD1306277 Protein, CILD14 Protein, FAP59 Protein, coiled-coil domain containing 39 Protein, flagellar associated protein Protein, Ccdc39 Protein, CCDC39 Protein, ccdc39 Protein, FAP59 Protein
- Background
- Coiled-coil domain-containing protein 39,FUNCTION: Required for assembly of dynein regulatory complex (DRC) and inner dynein arm (IDA) complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella. Probably acts together with CCDC40 to form a molecular ruler that determines the 96 nanometer (nm) repeat length and arrangements of components in cilia and flagella. Not required for outer dynein arm complexes assembly. {ECO:0000250|UniProtKB:A8IQT2, ECO:0000250|UniProtKB:Q9UFE4}.
- Molecular Weight
- 110.1 kDa
- UniProt
- Q9D5Y1
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