NDC80 Protein (AA 1-642) (Strep Tag)
-
- Target See all NDC80 Proteins
- NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-642
- Origin
- Mouse
-
Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This NDC80 protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
-
MKRSSVSTCG AGRLSMQELR TLDLNKPGLY TPQTKERSTF GKLSTHKPTS ERKVSIFGKR TSGHGSRNSQ LGIFSSSEKI KDPRPLNDKA FIQQCIRQLY EFLTENGYVY SVSMKSLQAP STKEFLKIFA FLYGFLCPSY ELPGTKCEEE VPRIFKALGY PFTLSKSSMY TVGAPHTWPH IVAALVWLID CIKIDTAMKE SSPLFDDGQL WGEETEDGIK HNKLFLEYTK KCYEKFMTGA DSFEEEDAEL QAKLKDLYKV DASKLESLEA ENKALNEQIA RLEEEREREP NRLMSLKKLK ASLQADVQNY KAYMSNLESH LAVLKQKSNS LDEEIGRVEQ ECETVKQENT RLQSIVDNQK YSVADIERIN HEKNELQQTI NKLTKDLEAE QQQMWNEELK YARGKEAIEA QLAEYHKLAR KLKLIPKGAE NSKGYDFEIK FNPEAGANCL VKYRTQVYAP LKELLNESEE EINKALNKKR HLEDTLEQLN TMKTESKNTV RMLKEEIQKL DDLHQQAVKE AEEKDKKSAS ELESLEKHKH LLESGVNDGL SEAMDELDAV QREYQLTVKT TTEERRKVEN NLQRLLEMVA THVGSLEKHL EEENAKADRE YEEFMSEDLL ENIREMAEKY KRNAAQLKAP DK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product NDC80 Protein
-
-
- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
-
- Target
- NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))
- Alternative Name
- Ndc80 (NDC80 Products)
- Synonyms
- CG9938 Protein, Dmel\\CG9938 Protein, Hec1 Protein, NDC80 Protein, dNdc80 Protein, dmNdc80 Protein, hec1 Protein, KNTC2 Protein, ndc10 Protein, tid3 Protein, 2610020P18Rik Protein, AV139730 Protein, HEC Protein, HEC1 Protein, Kntc2 Protein, kntc2l Protein, wu:fb52e02 Protein, zgc:111801 Protein, HsHec1 Protein, TID3 Protein, hsNDC80 Protein, kntc2 Protein, NDC80, kinetochore complex component Protein, CG9938 gene product from transcript CG9938-RA Protein, spindle pole body protein Ndc80 Protein, kinetochore protein Ndc80 Protein, NDC80 homolog, kinetochore complex component (S. cerevisiae) Protein, NDC80 kinetochore complex component Protein, NDC80 kinetochore complex component S homeolog Protein, NDC80 Protein, Ndc80 Protein, ndc80 Protein, ndc80.S Protein
- Background
- Kinetochore protein NDC80 homolog (Kinetochore protein Hec1) (Kinetochore-associated protein 2),FUNCTION: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules. {ECO:0000250|UniProtKB:O14777}.
- Molecular Weight
- 74.0 kDa
- UniProt
- Q9D0F1
- Pathways
- Maintenance of Protein Location
-