KIF18A Protein (AA 1-886) (Strep Tag)
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- Target See all KIF18A Proteins
- KIF18A (Kinesin Family Member 18A (KIF18A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-886
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This KIF18A protein is labelled with Strep Tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- Sequence
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MSGTEEDLCH RMKVVVRVRP ENTKEKAVQF CKVVHVVDKH ILSFDPKQEE ISFFHRKKTT NFDITKRQNK DLKFVFDAVF DETSTQMEVF EHTTKPILHS FLNGYNCTVF AYGATGSGKT HTMLGSAAEP GVMYLTMLDL FKCIDEIKEE KECSTAVSYL EVYNEQIRDL LTNSGPLAVR EDSQKGVVVQ GLTLHQPKSS EEILQLLDNG NKNRTQHPTD VNAVSSRSHA VFQIYLRQQD KTASINQNVR IAKMSLIDLA GSERASVSGA KGSRFVEGTN INKSLLALGN VINALANTKR RNQHIPYRNS KLTRLLKDSL GGNCQTIMIA AVSPSSLFYD DTYNTLKYAN RAKEIKSSLK SNVLNLNSHI SQYVKICNMQ KAEILMLKEK LKAYEEQKAL SDRNDCAKLV HSNPEDRETE RFQEILNCLF QNREGIRQEY LKLEMLLKAN ALKSSYHQQC HKQIEMMCSE DKVEKATCKR DHRLEKLKTN SCFLEKKKEE VSKQFDENTN WLHRVENEMR LLGQNGDIPE ALNKELHCHH LHLQNKELKT QMAHMTALAC LQEQQHKQTE AVLNALLPVL RKQYWKLKET GLSNAAFDSD FKDIEHLVER KKVVAWADQT NEHSNRNDLP GISLLMTFPQ LEPIQSISCC TSVSDPNVLK LTPQRRTRRK IIPSPLKVQH TQKSALSEST QLNDSFSKEL QPIVYTPEDC KKAQDLFPSL TRTSSQSANV MNDNSQKALC RIESPLSRTE CKQGLYSTST LCDSIRGLKN KWPEQEPLAS SKSSVHRIES SSFSTKDSMP ESAGVPSYMA MTTAAKRKWK QMSSTSNASI KSDESCGFAK RIRRDNSSVK PMQENRLKVG YKRNTNKTNS NMLRKFRRNT SKENVQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product KIF18A Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- KIF18A (Kinesin Family Member 18A (KIF18A))
- Alternative Name
- Kif18a (KIF18A Products)
- Synonyms
- MGC121839 Protein, MS-KIF18A Protein, AU024633 Protein, B130001M12Rik Protein, RGD1561740 Protein, kinesin family member 18A L homeolog Protein, kinesin family member 18A Protein, kif18a.L Protein, KIF18A Protein, kif18a Protein, Kif18a Protein
- Background
- Kinesin-like protein KIF18A,FUNCTION: Microtubule-depolymerizing kinesin which plays a role in chromosome congression by reducing the amplitude of preanaphase oscillations and slowing poleward movement during anaphase, thus suppressing chromosome movements. May stabilize the CENPE-BUB1B complex at the kinetochores during early mitosis and maintains CENPE levels at kinetochores during chromosome congression (By similarity). {ECO:0000250}.
- Molecular Weight
- 100.9 kDa
- UniProt
- Q91WD7
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