ANAPC4 Protein (AA 1-807) (Strep Tag)
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- Target See all ANAPC4 Proteins
- ANAPC4 (Anaphase Promoting Complex Subunit 4 (ANAPC4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-807
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This ANAPC4 protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
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MLRFPTCFPS FRVVGEKQLP QEIIFLAWSP KRDLIALANT TGEVLLHRLA SFHRVWSFPP NESTGKEVTC LAWRPDGKLL AFALADTKKI ILCDVEKPES LHSFSVEAPV SCMHWTEVTV ESSVLTSFYN AEDESNLLLP KLPTLPKNYN STSKIFSEEN SDEIIKLLGD VRLNILVLGG SSGFIELYAY GMFKIARVTG IAGTCIALCL SSDLKSLSVV TEVSSGGESE VSYFQLETNL LYSFLPEVTR MARKFTHISA LLQYINLSLT CMCEAWEEIL MQMDSRLTKF VQEKPTTTSV QDEFMHLLLW GKASAELQTL LMNQLTVKGL KKLGQSIESS YSSIQKLVIS HLQSGSESLL YHLSELKGMA SWKQKYEPLG LDAAGIEDAI TAVGSFILKA NELLQVIDSS MKNFKAFFRW LYVAMLRMTE DHVLPELNKM TQKDITFVAE FLTEHFNEAP DLYNRKGKYF NVERVGQYLK DEDDDLVSPP NTEGNQWYDF LQNSTHLKES PLLFPYYPRK SLHFVKRRME NVIDQCLQKP ADVIGRSMNQ AICIPLYKDA RSMDCARRLL KFPFLWNNKT SNLHYLLFTI LEDSVYKMCI LRRHTDISQS VSNGLIGIKF GSFTSASADK VRRSSYSCLD AQFYDDETVT VILKDSMGRE GRDRILVQLS LSLVYNSEDS DEYEFTGSYS TRLDEQGSII PTRTMHFEKH WRLLESMRAQ YVAGNGLRKV SCVLSSNLRH VRVFEMDIDD EWEIDESSDD EEEAGGKPVK IKEEVLSESE TEAHQDAAAL DPDVVIKVEK LDPELDS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product ANAPC4 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ANAPC4 (Anaphase Promoting Complex Subunit 4 (ANAPC4))
- Alternative Name
- Anapc4 (ANAPC4 Products)
- Synonyms
- MGC81278 Protein, DDBDRAFT_0186409 Protein, DDBDRAFT_0234268 Protein, DDB_0186409 Protein, DDB_0234268 Protein, APC4 Protein, ANAPC4 Protein, 2610306D21Rik Protein, D5Ertd249e Protein, F18E5.150 Protein, F18E5_150 Protein, anaphase promoting complex subunit 4 Protein, anaphase promoting complex subunit 4 L homeolog Protein, Transducin/WD40 repeat-like superfamily protein Protein, Anapc4 Protein, anapc4.L Protein, ANAPC4 Protein, anapc4 Protein, APC4 Protein
- Background
- Anaphase-promoting complex subunit 4 (APC4) (Cyclosome subunit 4),FUNCTION: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity). {ECO:0000250}.
- Molecular Weight
- 91.7 kDa
- UniProt
- Q91W96
- Pathways
- M Phase
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