DLGAP5 Protein (AA 1-808) (Strep Tag)
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- Target See all DLGAP5 Proteins
- DLGAP5 (Discs, Large (Drosophila) Homolog-Associated Protein 5 (DLGAP5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-808
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This DLGAP5 protein is labelled with Strep Tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- Sequence
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MLVSRFASRF RKDSSTEMVR TNLAHRKSLS QKENRHRVYE RNRHFGLKDV NIPLEGRELG NIHETSQDLS PEKASSKTRS VKMVLSDQRK QLLQKYKEEK QLQKLKEQRE KAKRGVFKVG LYRPAAPGFL VTDQRGAKAE PEKAFPHTGR ITRSKTKEYM EQTKIGSRNV PKATQSDQRQ TSEKQPLDRE RKVMQPVLFT SGKGTESAAT QRAKLMARTV SSTTRKPVTR ATNEKGSERM RPSGGRPAKK PEGKPDKVIP SKVERDEKHL DSQTRETSEM GPLGVFREVE SLPATAPAQG KERKSFAPKH CVFQPPCGLK SYQVAPLSPR SANAFLTPNC DWNQLRPEVF STTTQDKANE ILVQQGLESL TDRSKEHVLN QKGASTSDSN HASVKGVPCS EGSEGQTSQP PHDVPYFRKI LQSETDRLTS HCLEWEGKLD LDISDEAKGL IRTTVGQTRL LIKERFRQFE GLVDNCEYKR GEKETTCTDL DGFWDMVSFQ VDDVNQKFNN LIKLEASGWK DSNNPSKKVL RKKIVPGRTS KAKQDDDGRA AARSRLAAIK NAMKGRPQQE VQAHAAAPET TKEVDKIVFD AGFFRIESPV KSFSVLSSER RSQRFGTPLS ASKVVPEGRA AGDLLRQKMP LKKPDPQSSK SEHVDRTFSD GLESRCHVED TPCPGEQDSS DIEHDVNKIN VKMDCFSVET NLPLPAGDAN TNQKEAISAV EGASTAVTSQ DLLMSNPETN TSSQSNTSQE EAEASQSVLL HKSLTSECHL LEPPGLSCTS PCTREETRQP DRSRQFSFGG DLILFSPL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product DLGAP5 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- DLGAP5 (Discs, Large (Drosophila) Homolog-Associated Protein 5 (DLGAP5))
- Alternative Name
- Dlgap5 (DLGAP5 Products)
- Synonyms
- DLG7 Protein, dlg7 Protein, sb:cb647 Protein, zgc:92146 Protein, wu:fc96g08 Protein, wu:fe11c02 Protein, HURP Protein, C77459 Protein, C86398 Protein, Dap-5 Protein, Dlg7 Protein, Hurp Protein, mKIAA0008 Protein, DLG associated protein 5 Protein, discs, large (Drosophila) homolog-associated protein 5 Protein, DLGAP5 Protein, dlgap5 Protein, Dlgap5 Protein
- Background
- Disks large-associated protein 5 (DAP-5) (Discs large homolog 7) (Disks large-associated protein DLG7) (Hepatoma up-regulated protein homolog) (HURP),FUNCTION: Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells (By similarity). {ECO:0000250|UniProtKB:Q15398}.
- Molecular Weight
- 90.2 kDa
- UniProt
- Q8K4R9
- Pathways
- M Phase
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