POGZ Protein (AA 1-1409) (Strep Tag)
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- Target See all POGZ Proteins
- POGZ (Pogo Transposable Element with ZNF Domain (POGZ))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1409
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This POGZ protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MADTDLFMEC EEEELEPWQK ISDVIEDSVV EDYNSVDKTT SVSVSQQPVS APVPIAAHAS VAGHLSTSTT VSNSGAQNSD STKKTLVTLI ANNNAGNTLV QQGGQPLILT QNPAPGLGTM VTQPVLRPVQ VMQNANHVTS SPVASQPIFI TTQGFPVRNV RPVQNAMNQV GIVLNVQQGQ TVRPITLVPA PGTQFVKPTV GVPQVFSQMT PVRPGSTMPV RPTTNTFTTV IPATLTIRST VPQSQSQQTK STPSTSTTPT ATQPTSLGQL AGQPPGQSNQ TSNPKLAPSF PSPPAVSIAS FVTVKRPGVT GENSNEVAKL VNTLNTVPSL GQSPGPVVVS NNSSAQRTSG PESSVKVTSS IPVFDLQDGG RKICPRCNAQ FRVTEALRGH MCYCCPEMVE YQKKGKSLDA EPSVPSAAKP SSPEKTAPVT STPSSTPIPA LSPPTKVPEP NENAGDAVQT KLIMLVDDFY YGRDGGKAAQ LTSFPKVATS FRCPHCTKRL KNNIRFMNHM KHHVELDQQN GEVDGHTICQ HCYRQFSTPF QLQCHLENVH SPYESTTKCK ICEWAFESEP LFLQHMKDTH KPGEMPYVCQ VCQYRSSLYS EVDVHFRMIH EDTRHLLCPY CLKVFKNGNA FQQHYMRHQK RNVYHCNKCR LQFLFAKDKI EHKLQHHKTF RKPKQLEGLK PGTKVTIRAS RGQPRTVPVS SNDAPSGTLQ EAAALTSTDP LPVFLYPPVQ RNIQKRAVRK MSVMGRQTCL ECSFEIPDFP NHFPTYVHCS LCRYSTCCSR AYANHMINNH VPRKSPKYLA LFKNSVSGIK LACTSCTFAT SVGDAMAKHL VFNPSHRSSN ILPRGLSWMS HLRPGQASER VFDWSMKNTY LPPPLVPNKA ATVKPVGVTP AEPQELAGPV LQALPSPAST ATPPATPTHP QPSALPPSAT EGTECLNVSE QEEGSPVTQD PEPASGGGGG SGVGKKEQLS VKKLRVVLFA LCCNTEQAAE HFRNPQRRIR RWLRRFQASQ GENLEGKYLS FEAEEKLAEW VLIQREQQLP VNEETLFQKA TKIGRSLEGG FKISYEWAVR FMLRHHLTPH ARRAVAHTLP KHVAENAGLF IEFVQRQIHN QDLPLSMIVA IDEISLFLDT EVLSSDDRKE NALQTVGTGE PWCDVVLAIL ADGTVLPTLV FFRGQANRFA NVPDSILLEA KDSGYSDDEI MELWSTRVWK KHTACQHSKS MLVMDCHRTH LSEEVLALLS ASSTLPAVVP AGCSSKIQPL DVCIKRTVKN FLHKKWKEQA REMADAACDS DVLLQLVLVW LGEVLGVIGD SPELVQRSFL VASVLPGPDG NVNSPTRNAD MQEELIASLE EQLKLNGEQS EEHSASAPRP RSSPEETVEP ESLHQLFEGE SETESFYGFE EADLDLMEI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product POGZ Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- POGZ (Pogo Transposable Element with ZNF Domain (POGZ))
- Alternative Name
- Pogz (POGZ Products)
- Synonyms
- POGZ Protein, ZNF280E Protein, ZNF635 Protein, ZNF635m Protein, 9530006B08Rik Protein, AU015913 Protein, AU044539 Protein, pogo transposable element with ZNF domain Protein, pogo transposable element derived with ZNF domain Protein, POGZ Protein, pogz Protein, Pogz Protein
- Background
- Pogo transposable element with ZNF domain,FUNCTION: Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms (By similarity). Promotes the repair of DNA double-strand breaks through the homologous recombination pathway (By similarity). {ECO:0000250|UniProtKB:Q7Z3K3}.
- Molecular Weight
- 154.9 kDa
- UniProt
- Q8BZH4
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