RIMBP2 Protein (AA 1-1072) (Strep Tag)
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- Target See all RIMBP2 Proteins
- RIMBP2 (RIMS Binding Protein 2 (RIMBP2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1072
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This RIMBP2 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MREAAERRQQ LELEHEQALA FLNAKQQEIQ LLQQAQVEAK KEHEGAVQLL ESKVRELEEK CRVQSEQFNL LSRDLEKFRQ HTGSIDLLGS SSVALLDVPL APGKPFPQYM NGLATSIHKG HEGPTGHYSV IGDYIPLSGD KLESPCVKPS FLLRSSSPRC RFESEMDNDR NSNNSKQSSS GKVHLCVARY SYNPFDGPNE NPEAELPLTA GKYLYVYGDM DEDGFYEGEL LDGQRGLVPS NFVDFIQDNE SRLAGTLGSE QDQNFLNHSG ISLERDSILH LHSPTQVDSG ITDNGGGTLD VNIDDIGEDT VPYPRKITLI KQLAKSVIVG WEPPAVPPGW GTVSSYNVLV DKETRMSLAL GRRTKALIEK LNTAACTYRI SVQCVTSRGN SDELQCTLLV GKDVVVAPSQ LRVDNITQIS AQLSWLPTNS NYSHIIFLNE EELDIVKAAR YKYQFFNLRP NMAYKVKVLA QPHQMPWQLP LEQREKKEAC VEFSTLPAGP PAPPQDVTVH AGATAASVQV SWKPPALTPT GLSNGANVTG YGVYAKGQRV AEVIAPTADG TAVELIRLRS LEAKAVSVRT LSVQGESMDS ALAAIPPDLL VPPAPHPRTA PPPKPLASDM DTKDQHLGPH VKVDESWEQS RSPGPAHGHM LEPPDMHSAG PGRRSPSPSR ILPQPQGAPV STTVAKAMAR EAAQRVAESN RLEKRSLFLE QSSAGQYTNS DEEDGYASPE VKRRGTSVDD FLKGSELGKQ PHCCHGDEYH TESSRGSDLS DIMEEDEEEL YSEMQLEDGG RRRPSGTSHN ALKILGNSTL MGRADRMEHV SRRYSHSGGG SHRHRPAMAP SIDEYTGRDH LSPDFYDESE TDPGAEELPA RIFVALFDYD PLTMSPNPDA AEEELPFKEG QIIKVYGDKD ADGFYRGETC ARLGLIPCNM VSEIHADDEE MMDQLLRQGF LPLNTPVEKI ERSRRSGRGH SVPTRRMVAL YDYDPRESSP NVDVEAELPF CTGDIITVFG EIDEDGFYYG ELNGQKGLVP SNFLEEVPDD VEVHLSDAPP HYSHDPPMRS KAKRKKSVHF TP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product RIMBP2 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- RIMBP2 (RIMS Binding Protein 2 (RIMBP2))
- Alternative Name
- Rimbp2 (RIMBP2 Products)
- Synonyms
- RBP2 Protein, RIM-BP2 Protein, A930033C01Rik Protein, mKIAA0318 Protein, Rbp2 Protein, RIMS binding protein 2 Protein, RIMBP2 Protein, Rimbp2 Protein
- Background
- RIMS-binding protein 2 (RIM-BP2),FUNCTION: Plays a role in the synaptic transmission as bifunctional linker that interacts simultaneously with RIMS1, RIMS2, CACNA1D and CACNA1B. {ECO:0000250}.
- Molecular Weight
- 118.3 kDa
- UniProt
- Q80U40
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