TRAF3IP1 Protein (AA 1-625) (Strep Tag)
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- Target See all TRAF3IP1 Proteins
- TRAF3IP1 (TNF Receptor-Associated Factor 3 Interacting Protein 1 (TRAF3IP1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-625
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This TRAF3IP1 protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
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MNAAVVRRTQ EALGKVIRRP PLTEKLLNKP PFRYLHDIIT EVIRITGFMK GLYTDAEMKS ENVKDKDAKI SFLQKAIDVV MMVSGEPLAA KPARIVAGHE PERTNELLQL IGKCCLSKLS SDEAVKRVLA GDKGDSRGRA QRTSKAQEPN NKSGKEEESR IHKEDKRSSE AKERSASAEH KQKEELKEDS KPREKERDKE KAKEADRDRH RDPDRDRNRD GEREKARARA KDRDRNNRDR DREAERDRER DRRSEGGKEK ERVKDRDRDR DKGRDRERRK SKNGEHTRDP DREKSRDADK PEKKSSSSGE ISRKLSDGSF KDVKAEMEAD ISVGASRSST LKPSKRRSKH SLEGDSPSDA EVEAGPAGQD KPEVMENAEV PSELPSSLRR IPRPGSARPA PPRVKRQEST ETLVVDRSGS GKTVSSVIID SQNSDNEDDE QFVVEAAPQL SEIADIDMVP SGELEDEEKH GGLVKKILET KKDYEKLQQS LKPGEKERSL IFESAWKKEK DIVSKEIEKL RVSIQTLCKS ALPLGKIMDY IQEDVDAMQN ELQLWHSENR QHAEALSQEQ SITDSAVEPL KAELSELEQQ IRDQQDKICA VKANILKNEE KIQKMVHSIN LSSRR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product TRAF3IP1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- TRAF3IP1 (TNF Receptor-Associated Factor 3 Interacting Protein 1 (TRAF3IP1))
- Alternative Name
- Traf3ip1 (TRAF3IP1 Products)
- Synonyms
- Elipsa Protein, zgc:63522 Protein, MGC184660 Protein, MIP-T3 Protein, MIPT3 Protein, 3930402D05Rik Protein, AU041749 Protein, TNF receptor-associated factor 3 interacting protein 1 Protein, TRAF3 interacting protein 1 Protein, traf3ip1 Protein, TRAF3IP1 Protein, Traf3ip1 Protein
- Background
- TRAF3-interacting protein 1 (Intraflagellar transport protein 54 homolog) (Microtubule-interacting protein associated with TRAF3) (MIP-T3),FUNCTION: Plays an inhibitory role on IL13 signaling by binding to IL13RA1. Involved in suppression of IL13-induced STAT6 phosphorylation, transcriptional activity and DNA-binding. Recruits TRAF3 and DISC1 to the microtubules (By similarity). Involved in epithelial morphogenesis and in the regulation of microtubule cytoskeleton organization. Is a negative regulator of microtubule stability, acting through the control of MAP4 levels (PubMed:26487268). Involved in ciliogenesis (PubMed:21945076). {ECO:0000250|UniProtKB:Q8TDR0, ECO:0000269|PubMed:21945076, ECO:0000269|PubMed:26487268}.
- Molecular Weight
- 71.0 kDa
- UniProt
- Q149C2
- Pathways
- Hedgehog Signaling
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