NBR1 Protein (AA 1-988) (Strep Tag)
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- Target See all NBR1 Proteins
- NBR1 (Neighbor of BRCA1 Gene 1 (NBR1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-988
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This NBR1 protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
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MEPQVTLNVT FKNETQSFLV SDPENTTWAD VEAMVKVSFD LNTIQIKYLD EENEEISINS QGEYEEALKM ANIKQGNQLQ MQVHEGYHVV DEALPKNVVE NQAAARTGKK PLAHYSSLVR VLGSDMKTTE EPAPEQCSSA PCDTDQPQDK PPDWFTSYLE MFREQVVKET VEKLEQRLQE KLVLQKPLLS SSPTEVSMPI SEETLFLPEN QFSWHIACSH CQKRIVGVRY QCSLCPSYNI CEDCEAGPYT HDTNHVLLKL RRPVVISSEP FFYSKYSAPR LPAALEQVRL QKQVDKNFVK AEKQRLRAEK KQRKAEVKEL KKQLKLHRKI HLWNSIHGLQ SPKSPLGRPE SLLQSNTLML PLQPCAPVMP TLSAAFVDEN LPDGTHLQPG TKFIKHWRMK NTGNVKWNTD TKLKFMWGNL TLASTEKKDV LVPCLKAGHV GVVSVEFIAP TLEGTYTSHW RLSHKGQQFG PRVWCSIIVD PFPSSESPDN VEGDRISSSK ADDFSCEQEE AFLLAEEEIP LGEVTKQTEG TGASASQKTR RAASERELYI PSVDLLTAQD LLSFELLDIN IVQELERVPH NTPVDMTPCM SPLPHDSPLI EKPGLGQIQE ESEGAGFKAP PDSTVSAKRK AETPASVEET EEDLSGTQFV CETVIRSLTL DAAPDHNPPC RQRSPQRELQ LYSTEGQQPL VLPGFCRKDS SLKFALPEEG PRGDEREEIV HIVEEEVVEE EEEVQDEEVQ SQSSASSEDY IIILPECFDT SRPLGDSMYS SALSQPGLER GAEGEPGIES GLEPTEARER LPERESQPQE QSISDILTTS QPLDTVPLVP EVAGLPAALS RSAPCGQCES SGVDSPGVDS PATMHEVPPA PDDIRGEPRG STGLANSRQR SCDHSRHHNG SSIAGGLVKG ALSVAASAYK ALFSGPPVTA QPIVSEDQTT ALMAHLFEMG FCDRQLNLRL LRKHNYNILQ VVTELLQVNN NDWYSHRY
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product NBR1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- NBR1 (Neighbor of BRCA1 Gene 1 (NBR1))
- Alternative Name
- Nbr1 (NBR1 Products)
- Synonyms
- fa99f08 Protein, wu:fa99f08 Protein, MGC69008 Protein, NBR1 Protein, 1A1-3B Protein, M17S2 Protein, mKIAA0049 Protein, Ca125 Protein, RGD1311421 Protein, neighbor of brca1 gene 1a Protein, neighbor of BRCA1 gene 1 L homeolog Protein, NBR1, autophagy cargo receptor Protein, neighbor of Brca1 gene 1 Protein, nbr1a Protein, nbr1.L Protein, NBR1 Protein, Nbr1 Protein
- Background
- Next to BRCA1 gene 1 protein (Membrane component chromosome 17 surface marker 2 homolog) (Neighbor of BRCA1 gene 1 protein),FUNCTION: Ubiquitin-binding autophagy adapter that participates in different processes including host defense or intracellular homeostasis (PubMed:31916398, PubMed:34374750). Possesses a double function during the selective autophagy by acting as a shuttle bringing ubiquitinated proteins to autophagosomes and also by participating in the formation of protein aggregates. Plays a role in the regulation of the innate immune response by modulating type I interferon production and targeting ubiquitinated IRF3 for autophagic degradation (By similarity). In response to oxidative stress, promotes an increase in SQSTM1 levels, phosphorylation, and body formation by preventing its autophagic degradation (PubMed:31916398). In turn, activates the KEAP1-NRF2/NFE2L2 antioxidant pathway (PubMed:31916398). Plays also non-autophagy role by mediating the shuttle of IL-12 to late endosome for subsequent secretion (PubMed:34374750). {ECO:0000250|UniProtKB:Q14596, ECO:0000269|PubMed:31916398, ECO:0000269|PubMed:34374750}.
- Molecular Weight
- 110.0 kDa
- UniProt
- P97432
- Pathways
- Autophagy
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