Retinoid X Receptor beta Protein (AA 1-520) (Strep Tag)
-
- Target See all Retinoid X Receptor beta (RXRB) Proteins
- Retinoid X Receptor beta (RXRB) (Retinoid X Receptor, beta (RXRB))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-520
-
Origin
- Mouse
-
Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This Retinoid X Receptor beta protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
-
MSWATRPPFL PPRHAAGQCG PVGVRKEMHC GVASRWRRRR PWLDPAAAAA AAGEQQALEP EPGEAGRDGM GDSGRDSRSP DSSSPNPLSQ GIRPSSPPGP PLTPSAPPPP MPPPPLGSPF PVISSSMGSP GLPPPAPPGF SGPVSSPQIN STVSLPGGGS GPPEDVKPPV LGVRGLHCPP PPGGPGAGKR LCAICGDRSS GKHYGVYSCE GCKGFFKRTI RKDLTYSCRD NKDCTVDKRQ RNRCQYCRYQ KCLATGMKRE AVQEERQRGK DKDGDGDGAG GAPEEMPVDR ILEAELAVEQ KSDQGVEGPG ATGGGGSSPN DPVTNICQAA DKQLFTLVEW AKRIPHFSSL PLDDQVILLR AGWNELLIAS FSHRSIDVRD GILLATGLHV HRNSAHSAGV GAIFDRVLTE LVSKMRDMRM DKTELGCLRA IILFNPDAKG LSNPGEVEIL REKVYASLET YCKQKYPEQQ GRFAKLLLRL PALRSIGLKC LEHLFFFKLI GDTPIDTFLM EMLEAPHQLA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product RXRB Protein
-
-
- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
-
- Target
- Retinoid X Receptor beta (RXRB) (Retinoid X Receptor, beta (RXRB))
- Alternative Name
- Rxrb (RXRB Products)
- Synonyms
- DAUDI6 Protein, H-2RIIBP Protein, NR2B2 Protein, RCoR-1 Protein, RXR-beta Protein, RXRbeta Protein, rxrb Protein, RXRB Protein, nr2b2 Protein, daudi6 Protein, rcor-1 Protein, h-2riibp Protein, NR2B2-A Protein, RXR Protein, RXRA Protein, etID309733.19 Protein, rxre Protein, wu:fb93c09 Protein, AL023085 Protein, Nr2b2 Protein, Rub Protein, rxrd Protein, unp286 Protein, retinoid X receptor beta Protein, retinoid X receptor beta S homeolog Protein, retinoid x receptor, beta a Protein, retinoid x receptor, beta b Protein, RXRB Protein, Rxrb Protein, rxrb.S Protein, rxrb Protein, rxrba Protein, rxrbb Protein
- Background
- Retinoic acid receptor RXR-beta (MHC class I regulatory element-binding protein H-2RIIBP) (Nuclear receptor subfamily 2 group B member 2) (Retinoid X receptor beta),FUNCTION: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE). {ECO:0000269|PubMed:1310259, ECO:0000269|PubMed:1312497}.
- Molecular Weight
- 55.9 kDa
- UniProt
- P28704
- Pathways
- Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
-