FPGT Protein (AA 1-590) (Strep Tag)
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- Target See all FPGT Proteins
- FPGT (Fucose-1-Phosphate Guanylyltransferase (FPGT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-590
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Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This FPGT protein is labelled with Strep Tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- Sequence
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MASLREATLR KLRRFSELRG KPVAAGEFWD VVAITAADEK QELAYKQQLS EKLKKRELPL GVQYHVFPDP AGTKIGNGGS TLCSLECLES LCGDKWNSLK VLLIHSGGYS QRLPNASALG KIFTALPLGE PIYQMLELKL AMYVDFPSNM RPGVLVTCAD DIELYSVGDS EYIAFDQPGF TALAHPSSLA VGTTHGVFVL HSDSSLQHGD LEYRQCYQFL HKPTIENMHR FNAVHRQRSF GQQNLSGGDT DCLPLHTEYV YTDSLFYMDH KSAKKLLDFY KSEGPLNCEI DAYGDFLQAL GPGATAEYTR NTSHVTKEES QLLDMRQKIF HLLKGTPLNV VVLNNSRFYH IGTLQEYLLH FTSDSALKTE LGLQSIAFSV SPSVPERSSG TACVIHSIVD SGCCVAPGSV VEYSRLGPEV SIGENCIISS SVIAKTVVPA YSFLCSLSVK INGHLKYSTM VFGMQDNLKN SVKTLEDIKA LQFFGVCFLS CLDIWNLKAT EKLFSGNKMN LSLWTACIFP VCSSLSESAT ASLGMLSAVR NHSPFNLSDF NLLSIQEMLV YKDVQDMLAY REHIFLEISS NKNQSDLEKS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product FPGT Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- FPGT (Fucose-1-Phosphate Guanylyltransferase (FPGT))
- Alternative Name
- Fpgt (FPGT Products)
- Synonyms
- DDBDRAFT_0186414 Protein, DDBDRAFT_0233144 Protein, DDB_0186414 Protein, DDB_0233144 Protein, FPGT Protein, DKFZp459M111 Protein, fpgt Protein, zgc:110694 Protein, GFPP Protein, 1700016E03Rik Protein, fucose-1-phosphate guanylyltransferase Protein, fucose-1-phosphate guanylyltransferase L homeolog Protein, fpgt Protein, FPGT Protein, fpgt.L Protein, Fpgt Protein
- Background
- Fucose-1-phosphate guanylyltransferase (EC 2.7.7.30) (GDP-L-fucose diphosphorylase) (GDP-L-fucose pyrophosphorylase),FUNCTION: Catalyzes the formation of GDP-L-fucose from GTP and L-fucose-1-phosphate (PubMed:14686921). Functions as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids (Probable). {ECO:0000269|PubMed:14686921, ECO:0000305|PubMed:14686921}.
- Molecular Weight
- 65.4 kDa
- UniProt
- G5E8F4
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