TBC1D2 Protein (AA 1-922) (Strep Tag)
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- Target See all TBC1D2 Proteins
- TBC1D2 (TBC1 Domain Family, Member 2 (TBC1D2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-922
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This TBC1D2 protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
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MEDAPERTPS SSESTQPPGL AREPEVVSPG DSEGCARPLD TVPKKLCGYL SKFGGKGPIK GWKCRWFFYD ERKCHLYYSR TAQDANPLDS IDLSSAVFDC KADAEEEGTF EIKTPSRVIT LKAATKQAML YWLQQLQMKR WEFHNSPPAL PATPAAALAE NGPTLHLKLE QEEAELEEFL CPVKTPTGLV GAAAALQPVP AVPSALQNIS LKHLGTEIQN TMHNIRGNKQ AQAAAHGPLV EDSPQGGEPQ SGEQPSISDP SLPEKEPEDP AKSAPRSSVP SGPTQKPKRQ SNTFPFFSDG LARSRTAQEK VAALEQQVLM LTKELKSQKE LVIILHKALE AAQQEKRASS AYLAATEDRD RLELVRHKVR QIAELNQRVE ALEQGRERLA HEAGLREQQV QALQQHVQLL MDKNHAKQQV ICKLTQKLTE DLAQPADATN GDFLSQQERM EHLKDDMEAY RTQNRFLNSE IHQVTKIWRK VAEKEKALLT KCAYLQARNC QVESKYLAGL RRLQEAAGAE AGDFPELLQQ LIQEALQWEA GEADSVGLSP VSEYDDYGFL TVPDYEMEDL KLLAKIQALE VRSHHLLAHE AVERPLRDRW ATLTELTPSA ELKQLLRAGV PREHRPRVWR WLVHRRVRHL QAPGCYQELL ARGRACEHPA ARQIELDLNR TFPTNKHFTC PTSSFPDKLR RVLLAFSWQN PTIGYCQGLN RLAAIALLVL EDEESAFWCL VAIVETILPA EYYSKTLTAS QVDQRVLQDL LSEKLPRLTA HLGQHRVDLS LITFNWFLVV FADSLISDIL LRVWDAFLYE GTKVVFRYAL AIFKYNEEAI LQLQDSLEIY QYLRFFTKTI CDSRKLMSIA FNDMNPFPMK QLRQLRAAHR ERLEAELREL ELLKVEYLQR RASLGRAPPE GCVSEDEGEG DS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product TBC1D2 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- TBC1D2 (TBC1 Domain Family, Member 2 (TBC1D2))
- Alternative Name
- Tbc1d2 (TBC1D2 Products)
- Synonyms
- PARIS-1 Protein, PARIS1 Protein, TBC1D2A Protein, A630005A06Rik Protein, BC022630 Protein, Gm1037 Protein, TBC1 domain family member 2 Protein, TBC1 domain family, member 2 Protein, TBC1D2 Protein, Tbc1d2 Protein
- Background
- TBC1 domain family member 2A,FUNCTION: Acts as a GTPase-activating protein for RAB7A. Signal effector acting as a linker between RAC1 and RAB7A, leading to RAB7A inactivation and subsequent inhibition of cadherin degradation and reduced cell-cell adhesion (By similarity). {ECO:0000250}.
- Molecular Weight
- 104.3 kDa
- UniProt
- B1AVH7
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