FRMPD4 Protein (AA 1-1320) (Strep Tag)
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- Target See all FRMPD4 Proteins
- FRMPD4 (FERM and PDZ Domain Containing 4 (FRMPD4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1320
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This FRMPD4 protein is labelled with Strep Tag.
- Application
- Western Blotting (WB), ELISA, SDS-PAGE (SDS)
- Sequence
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MDVFSFVKIP KLSSHRTKSS GWPPPSGTWG LNQVPPYGWE MMTNRDGRDY FINHMTQAIP FDDPRFDSCQ IIPPAPRKVE MRRDPVLGFG FVAGSEKPVV VRSVTPGGPS EGKLIPGDQI VMINDEAVSA APRERVIDLV RSCKESILLT VIQPYPSPKS AFISAAKKAR LKSNPVKVRF SEEVIINGQV SETVKDNSLL FMPNVLKVYL ENGQTKSFRF DCSTSIKDVI LTLQEKLSIK GIEHFSLMLE QRIEGAGTKL LLLHEQETLT QVTQRPSSHK MRCLFRISFV PKDPIDLLRR DPVAFEYLYV QSCNDVVQER FGPELKYDIA LRLAALQMYI ATVTTKQTQK ISLKYIEKEW GLETFLPSAV LQSMKEKNIK KALSHLVKAN QNLVPPGKKL SALQAKVHYL KFLSDLRLYG GRVFKATLVQ AEKRSEVTLL VGPRYGISHV INTKTNLVAL LADFSHVNRI EMFTEEESLV RVELHVLDVK PITLLMESSD AMNLACLTAG YYRLLVDSRR SIFNMANKKN AGTQDTGSEN KGKHNLLGPD WNCMPQMTTF IGEGEQEAQI TYIDSKQKTV EMTDSTLCPK EHRHLYIDNS YSSDELNQPL TQPGDAPCEA DYRSLAQRSL LTLSGPDTLK KAQESPRGAK VSFIFGDLAL DDGMSPPTIG YERMLEENPE MLEKQRNLYI SSANDMKNLD LTPDTDSIQF VANSVYANIG DVKNFEAPEG IEEPLLHDIC YAENTDDAED EDEVSCEEDL VVGEMNQPAI LDLSGSSDDI IDLTTLPPPE GDDNEDDFLL RSLNMAIAAP PPGFRDSSDE EDTQSQATSF HEDKEQGSSL QNEEIPVSLI DAVPTSAEGK CEKGLDPAVV STLEALEALS EEQQKSENSG VAILRAYSPE SSSDSGNETN SSEMTEGSEL AAAQKQSESL SRMFLATHEG YHPLAEEQTE FPTSKAPSVG LPPKSSHGLA ARPATDLPPK VVPSKQILHS DHMEMEPETM ETKSVTDYFS KLHMGSVAYS CTSKRKSKLP EGEGKSPLSG NIPGKKQQGT KIAEIEEDTK GKAGTVSSRD NPHLSTFNLE RTAFRKDSQR WYVASDGGVV EKSGMEAPAM KVFPRGPGLG NREAEGKEDG TVEGGADDAS VLGQGDRFLT DMACVASAKD LDNPEDTDSP SCDHATKLSE AEDNVARLCD YHLAKRMSSL QSEGHFSLQS SQGSSVDTGC GPGSSSSACA TPVESPLCPS MGKHMIPDAS GKGGRYISPE ERAPGHPNHG ATFEELHPQT EGMCPRMTVP ALHTAINADP LFGTLRDGCH RLPKIKETTV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product FRMPD4 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- FRMPD4 (FERM and PDZ Domain Containing 4 (FRMPD4))
- Alternative Name
- Frmpd4 (FRMPD4 Products)
- Synonyms
- RGD1566031 Protein, PDZD10 Protein, PDZK10 Protein, Gm196 Protein, PKAP1 Protein, Pdzd10 Protein, Pdzk10 Protein, Preso1 Protein, FERM and PDZ domain containing 4 Protein, Frmpd4 Protein, FRMPD4 Protein, frmpd4 Protein
- Background
- FERM and PDZ domain-containing protein 4 (PDZ domain-containing protein 10) (PSD-95-interacting regulator of spine morphogenesis) (Preso),FUNCTION: Positive regulator of dendritic spine morphogenesis and density. Required for the maintenance of excitatory synaptic transmission. Binds phosphatidylinositol 4,5-bisphosphate. {ECO:0000250|UniProtKB:Q14CM0}.
- Molecular Weight
- 145.0 kDa
- UniProt
- A2AFR3
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