CDC27 Protein (AA 1-825) (Strep Tag)
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- Target See all CDC27 Proteins
- CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-825
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This CDC27 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MTVLQEPVQA AIWQALNHYA YRDAVFLAER LYAEVHSEEA LFLLATCYYR SGKAYKAYRL LKGHSCTTPQ CKYLLAKCCV DLSKLAEGEQ ILSGGVFNKQ KSHDDLVTEF GDSACFTLSL LGHVYCKTDR LAKGSECYQK SLSLNPFLWS PFESLCEIGE KPDPDQTFKL TSLQNFSSCL PNTCTTLVSN HSLSHRQPET VLTETPQDTI ELNRLNLESS NSKYSLNTDS SVSYIDSTVI SPDNVPLGPG TAILSKQVQN KPKTGRSLLG GPTALSPLTP SFGILPLETP SPGDGSYLQN YTNTPSVIDV APTGAPTKKS VARMGQTGTK SVFSQSGNSR EVTPVLVAQT QSSGPQTSTT PQVLSPTITS PPNALPRRSS RLFTSDSSTT KENSKKLKMK FPPKIPNRKT KSKTNKGGLT QPSINDSLEI TKLDSSIISE GKITTVTPQI QAFNLQKAAA EGLMSLLREM GKGYLALCSY NCKEAINILS HLPSHHYSTG WVLCQIGRAY FELSEYMQAE RIFSEVRRIE SFRVEGMEIY STTLWHLQKD VALSVLSKDL TDMDKNSPEA WCAAGNCFSL QREHDIAIKF FQRAIQVDPN YAYAYTLLGH EFVLTEELDK ALACFRNAIR VNPRHYNAWY GLGMIYYKQE KFSLAEMHFQ KALDINPQSS VLLCHIGVVQ HALKKSEKAL DTLNKAIVID PKNPLCKFHR ASVLFANEKY KSALQELEEL KQIVPKESLV YFLIGKVYKK LGQTHLALMN FSWAMDLDPK GANNQIKEAI DKRYLPDDEE PITQEEQIMG TDESQESSMT DADDTQLHAA ESDEF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product CDC27 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
- Alternative Name
- Cdc27 (CDC27 Products)
- Synonyms
- ANAPC3 Protein, APC3 Protein, CDC27Hs Protein, D0S1430E Protein, D17S978E Protein, HNUC Protein, NUC2 Protein, wu:fb54g06 Protein, wu:fc88c12 Protein, AI452358 Protein, BC023187 Protein, CDC27 Protein, NV10210 Protein, cell division cycle 27 Protein, cell division cycle 27 S homeolog Protein, cell division cycle protein 27 homolog Protein, anaphase-promoting complex subunit Apc3 Protein, CDC27 Protein, cdc27 Protein, Cdc27 Protein, cdc27.S Protein, LOC100123129 Protein, nuc2 Protein
- Background
- Cell division cycle protein 27 homolog,FUNCTION: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity). {ECO:0000250}.
- Molecular Weight
- 91.8 kDa
- UniProt
- A2A6Q5
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