T-Bet Protein (AA 1-530) (Strep Tag)

Catalog No. ABIN3130231

Product Details

Target: T-Bet

Protein Type: Recombinant

Protein Characteristics: AA 1-530

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This T-Bet protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MGIVEPGCGD MLTGTEPMPS DEGRGPGADQ QHRFFYPEPG AQDPTDRRAG SSLGTPYSGG ALVPAAPGRF LGSFAYPPRA QVAGFPGPGE FFPPPAGAEG YPPVDGYPAP DPRAGLYPGP REDYALPAGL EVSGKLRVAL SNHLLWSKFN QHQTEMIITK QGRRMFPFLS FTVAGLEPTS HYRMFVDVVL VDQHHWRYQS GKWVQCGKAE GSMPGNRLYV HPDSPNTGAH WMRQEVSFGK LKLTNNKGAS NNVTQMIVLQ SLHKYQPRLH IVEVNDGEPE AACSASNTHV FTFQETQFIA VTAYQNAEIT QLKIDNNPFA KGFRENFESM YASVDTSVPS PPGPNCQLLG GDPFSPLLSN QYPVPSRFYP DLPGQPKDMI SQPYWLGTPR EHSYEAEFRA VSMKPTLLPS APGPTVPYYR GQDVLAPGAG WPVAPQYPPK MSPAGWFRPM RTLPMDPGLG SSEEQGSSPS LWPEVTSLQP EPSDSGLGEG DTKRRRISPY PSSGDSSSPA GAPSPFDKET EGQFYNYFPN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: T-Bet

Alternative Name: Tbx21 (T-Bet Products)

Synonyms: T-PET Protein, T-bet Protein, TBET Protein, TBLYM Protein, TBT1 Protein, Tbet Protein, Tblym Protein, T-box 21 Protein, TBX21 Protein, Tbx21 Protein

Background: T-box transcription factor TBX21 (T-box protein 21) (T-cell-specific T-box transcription factor T-bet) (Transcription factor TBLYM),FUNCTION: Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs. Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation (PubMed:10761931, PubMed:17923685, PubMed:21095589). Can activate Th1 genes also via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells (PubMed:27292648). Inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC (PubMed:21151104). Inhibits the Th2 cell lineage commitment by suppressing the production of Th2 cytokines, such as IL-4, IL-5, and IL- 13, via repression of transcriptional regulators GATA3 and NFATC2 (PubMed:15662016, PubMed:21690296, PubMed:23616576). Protects Th1 cells from amplifying aberrant type-I IFN response in an IFN-gamma abundant microenvironment by acting as a repressor of type-I IFN transcription factors and type-I IFN- stimulated genes (PubMed:28623086). Acts as a regulator of antiviral B-cell responses, controls chronic viral infection by promoting the antiviral antibody IgG2a isotype switching and via regulation of a broad antiviral gene expression program (PubMed:27430722). {ECO:0000269|PubMed:10761931, ECO:0000269|PubMed:15662016, ECO:0000269|PubMed:17923685, ECO:0000269|PubMed:21095589, ECO:0000269|PubMed:21151104, ECO:0000269|PubMed:21690296, ECO:0000269|PubMed:23616576, ECO:0000269|PubMed:27292648, ECO:0000269|PubMed:27430722, ECO:0000269|PubMed:28607488, ECO:0000269|PubMed:28623086}.

Molecular Weight: 57.9 kDa

UniProt: Q9JKD8