HPS6 Protein (AA 1-805) (Strep Tag)
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- Target See all HPS6 Proteins
- HPS6 (Hermansky-Pudlak Syndrome 6 (HPS6))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-805
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This HPS6 protein is labelled with Strep Tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- Sequence
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MKRAGTLRLL SDLSNFTGAA RLRELLAGDP AVLVRCSPDG RHLLLLRPPG SPAPQLLVAV RGPGLPLERA WPEGDPSPLD VFFVPWLARP ALILVWESGL AEVWGVGMEP GWKLLQSTEL CPDGGARVMA VAATRGRLVW CEERQPGVKD QPEQLSTAFS HRVCFKTLET SGEAGTKLGC THILLHHCPL FGLIASRKDL FLVPTTNTWS GVAHLLLIWS PSKGKVIVAA PSLGLSHSKS LNPKQGDTWD FRTLLRGLPG FLSPREPLAV HTWAPSSQGL LLLDLKGKVS LVQCHGGTRT VGILQEAPVS LKGSAALGTF HGTLACVLGS TLELLDMSSG RLLEKKVLST DRVHLLEPPA PGMKNEEELE TRGALRLLSA LGLFCVCWET PQGLELPSDK DLVFEEACGY YQRRSLRGTQ LTPEELRHNS MFRAPQALAS ILQGHLPPST LLTTLRAELR DYRSIEQLKA QLVAGDDEEA GWTELAEHEV ARLLRTQLTG DQLAQFNTIF QALPTAAWGA TLQALQLQPD RSGRLRSQAP PDVWKKVLRA PTAGKEHPNG ILPPFELLCQ CLGQLEPQWL PPFVKLAQQQ GGPGWGAEGP SLPLYRRALA VLGEEGKRPE ALELELLLGS GRPKAVLQAV RQLIKKEEWE RALEAGLALD ASSPLLRSEI FKLLLAEFAQ HRRLDAHLPL LCRLCPPEVA PHELLLLLRT HLPDDEGTTP FPEPGAEPPL TVGLVRALLE QTGAQGRPSG PVQSTYEDIL WDPGTPPPTP PRGPTASLPA SDHPGQEAWV PPGQGLGAAD VGVHL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product HPS6 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- HPS6 (Hermansky-Pudlak Syndrome 6 (HPS6))
- Alternative Name
- Hps6 (HPS6 Products)
- Synonyms
- RP11-302K17.1 Protein, 5330434M19Rik Protein, BLOC2 Protein, ru Protein, Hsp6 Protein, HPS6, biogenesis of lysosomal organelles complex 2 subunit 3 Protein, HPS6 Protein, hps6 Protein, Hps6 Protein
- Background
- BLOC-2 complex member HPS6 (Hermansky-Pudlak syndrome 6 protein homolog) (Ruby-eye protein) (Ru),FUNCTION: May regulate the synthesis and function of lysosomes and of highly specialized organelles, such as melanosomes and platelet dense granules (By similarity). Acts as a cargo adapter for the dynein-dynactin motor complex to mediate the transport of lysosomes from the cell periphery to the perinuclear region. Facilitates retrograde lysosomal trafficking by linking the motor complex to lysosomes, and perinuclear positioning of lysosomes is crucial for the delivery of endocytic cargos to lysosomes, for lysosome maturation and functioning (PubMed:25189619). {ECO:0000250|UniProtKB:Q86YV9, ECO:0000269|PubMed:25189619}.
- Molecular Weight
- 87.3 kDa
- UniProt
- Q8BLY7
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