PHF15 Protein (AA 1-829) (Strep Tag)
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- Target See all PHF15 Proteins
- PHF15 (PHD Finger Protein 15 (PHF15))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-829
- Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This PHF15 protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
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MEEKRRKYSI SSDNSDTTDG HVTSTSASRC SKLPSSTKSG WPRQNEKKPS EVFRTDLITA MKIPDSYQLS PDDYYILADP WRQEWEKGVQ VPAGAEAIPE PVVRLLPPLK GPPTQMSPDS PTLGEGAHPD WPGGSRYDLD EIDAYWLELL NSELKEMEKP ELDELTLERV LEELETLCHQ NMAQAIETQE GLGIEYDEDV VCDVCRSPEG EDGNEMVFCD KCNVCVHQAC YGILKVPTGS WLCRTCALGV QPKCLLCPKR GGALKPTRSG TKWVHVSCAL WIPEVSIGCP EKMEPITKIS HIPASRWALS CSLCKECTGT CIQCSMPSCI TAFHVTCAFD RGLEMRTILA DNDEVKFKSL CQEHSDGGPR SEPTSEPVEP SQAVEDLEKV TLRKQRLQQL EENFYELVEP AEVAERLDLA EALVDFIYQY WKLKRRANAN QPLLTPKTDE VDNLAQQEQD VLYRRLKLFT HLRQDLERVR NLCYMVTRRE RTKHTICKLQ EQIFHLQMKL IEQDLCREPS GRRSKGKKND SKRKGREGPK GSSPEKKEKV KAGPESVLGQ LGLSTSFPID GTFFNSWLAQ SVQITAEDMA MSEWSLNSGH REDPAPGLLS EELLQDEETL LSFMRDPSLR PGDPARKARG RTRLPAKKKP SPLQDGPSAR TTPDKQPKKA WAQDGKGTQG PPMRKPPRRT SSHLPSSPAA GDCPVPATLE SPPPLASEIL DKTAPMASDL NVQVPGPTVS PKPLGRLRPP REMKVSRKSP GARSDAGTGL PSAVAERPKV SLHFDTEADG YFSDEEMSDS EVEAEDSGVQ RASREAGAEE VVRMGVLAS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product PHF15 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- PHF15 (PHD Finger Protein 15 (PHF15))
- Alternative Name
- Jade2 (PHF15 Products)
- Synonyms
- PHF15 Protein, JADE2 Protein, 1200017K05Rik Protein, AI480685 Protein, Jade2 Protein, mKIAA0239 Protein, jade family PHD finger 2 Protein, jade family PHD finger 2 L homeolog Protein, PHD finger protein 15 Protein, Jade2 Protein, JADE2 Protein, jade2.L Protein, GL50803_15559 Protein, jade2 Protein
- Background
- E3 ubiquitin-protein ligase Jade-2 (EC 2.3.2.27) (Jade family PHD finger protein 2) (PHD finger protein 15),FUNCTION: Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity (By similarity). Acts as a E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent proteasomal degradation of target protein histone demethylase KDM1A (PubMed:25018020). Also acts as a ubiquitin ligase E3 toward itself (PubMed:25018020). Positive regulator of neurogenesis (PubMed:25018020). {ECO:0000250|UniProtKB:Q9NQC1, ECO:0000269|PubMed:25018020}.
- Molecular Weight
- 92.2 kDa
- UniProt
- Q6ZQF7
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