TCF15 Protein (AA 1-195) (Strep Tag)
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- Target See all TCF15 Proteins
- TCF15 (Transcription Factor 15 (Basic Helix-Loop-Helix) (TCF15))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-195
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Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This TCF15 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MAFALLRPVG AHVLYPDVRL LSEDEENRSE SDASDQSFGC CEGLEAARRG PGPGSGRRAS NGAGPVVVVR QRQAANARER DRTQSVNTAF TALRTLIPTE PVDRKLSKIE TLRLASSYIA HLANVLLLGD AADDGQPCFR AAGGGKSAVP AADGRQPRSI CTFCLSNQRK GGSRRDLGGS CLKVRGVAPL RGPRR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product TCF15 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- TCF15 (Transcription Factor 15 (Basic Helix-Loop-Helix) (TCF15))
- Alternative Name
- Tcf15 (TCF15 Products)
- Synonyms
- EC2 Protein, PARAXIS Protein, bHLHa40 Protein, Meso1 Protein, bHLH-EC2 Protein, paraxis Protein, BHLHEC2 Protein, Paraxis Protein, TCF-15 Protein, meso1 Protein, par1 Protein, transcription factor 15 Protein, transcription factor 15 L homeolog Protein, transcription factor 15 (basic helix-loop-helix) Protein, TCF15 Protein, Tcf15 Protein, tcf15.L Protein, tcf15 Protein
- Background
- Transcription factor 15 (TCF-15) (Meso1) (Paraxis) (Protein bHLH-EC2),FUNCTION: Early transcription factor that plays a key role in somitogenesis, paraxial mesoderm development and regulation of stem cell pluripotency (PubMed:7597044, PubMed:8955271, PubMed:23395635, PubMed:32669716, PubMed:24038871). Essential for the mesenchymal to epithelial transition associated with somite formation (PubMed:7597044, PubMed:8955271, PubMed:24038871). Required for somite morphogenesis, thereby regulating patterning of the axial skeleton and skeletal muscles (PubMed:8955271). Required for proper localization of somite epithelium markers during the mesenchymal to epithelial transition (PubMed:24038871). Also plays a key role in regulation of stem cell pluripotency (PubMed:23395635, PubMed:32669716). Promotes pluripotency exit of embryonic stem cells (ESCs) by priming ESCs for differentiation (PubMed:23395635). Acts as a key regulator of self-renewal of hematopoietic stem cells (HSCs) by mediating HSCs quiescence and long-term self-renewal (PubMed:32669716). Together with MEOX2, regulates transcription in heart endothelial cells to regulate fatty acid transport across heart endothelial cells (PubMed:25561514). Acts by forming a heterodimer with another helix-loop-helix (bHLH) protein, such as TCF3/E12, that binds DNA on E-box motifs (5'-CANNTG-3') and activates transcription of target genes (PubMed:15226298, PubMed:23395635). {ECO:0000269|PubMed:15226298, ECO:0000269|PubMed:23395635, ECO:0000269|PubMed:24038871, ECO:0000269|PubMed:25561514, ECO:0000269|PubMed:32669716, ECO:0000269|PubMed:7597044, ECO:0000269|PubMed:8955271}.
- Molecular Weight
- 20.7 kDa
- UniProt
- Q60756
- Pathways
- Feeding Behaviour
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