ORC5 Protein (AA 1-435) (Strep Tag)
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- Target See all ORC5 Proteins
- ORC5 (Origin Recognition Complex, Subunit 5 (ORC5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-435
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Origin
- Mouse
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This ORC5 protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
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MSHLESMVLC REAQVSTLQS LFGERHHFSF PSIFIYGHTA SGKTYVTQTL LKTLELPHAF VNCVECFTSR LLLEQILNKL SHLNSSDAGC STEMTCETFN DFVQLFKQVT SAEHLKDQTV YIVLDKAEYL RDMEANLLPG LLRLQELTDR NVTVIFLSEI IWEKFRPNTG CFEPFVLYFP DYSIGNLQKI LSHDHPPEYS ADFYAAYINI LLGVFYTVCR DLKELRHLAV LNFPKYCEPV VKGEAGERDT RKLWRNIEPH LKKAMQTVYL REISSSQWEK LQKDNTDPGQ LKGLSAYTHM ELPYYSKFIL IAAYLASYNP ARTDKRFFLK HHGKIKKTNF LKKHEKTSNH LLGPKPFPLD RLLAILYSIV DSRVAPTANI FSQITSLVTL QLLTIVGHED QLNGPKYKCT VSLDFIRAIA RMVNFDIIKY LYDFL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product ORC5 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ORC5 (Origin Recognition Complex, Subunit 5 (ORC5))
- Alternative Name
- Orc5 (ORC5 Products)
- Synonyms
- Orc5l Protein, BG:DS00941.8 Protein, CG7833 Protein, D-orc5 Protein, DmORC5 Protein, DmOrc5 Protein, Dmel\\CG7833 Protein, ORC Protein, ORC5 Protein, br39 Protein, dORC5 Protein, l(2)34Df Protein, l(2)br39 Protein, orc5 Protein, orc5l Protein, wu:fb62d08 Protein, zgc:77827 Protein, ORC5L Protein, DDBDRAFT_0191826 Protein, DDBDRAFT_0233112 Protein, DDB_0191826 Protein, DDB_0233112 Protein, ORC5P Protein, ORC5T Protein, AL033327 Protein, MmORC5 Protein, origin recognition complex, subunit 5 Protein, Origin recognition complex subunit 5 Protein, origin recognition complex subunit 5 Protein, Orc5 Protein, orc5 Protein, ORC5 Protein, orcE Protein
- Background
- Origin recognition complex subunit 5,FUNCTION: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. {ECO:0000250|UniProtKB:O43913}.
- Molecular Weight
- 50.2 kDa
- UniProt
- Q9WUV0
- Pathways
- Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
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