TM9SF2 Protein (AA 29-663) (rho-1D4 tag)
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- Target See all TM9SF2 Proteins
- TM9SF2 (Transmembrane 9 Superfamily Member 2 (TM9SF2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 29-663
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This TM9SF2 protein is labelled with rho-1D4 tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
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GPRRSGAFYL PGLAPVNFCD EEKKSDECKA EIELFVNRLD SVESVLPYEY TAFDFCQASE GKRPSENLGQ VLFGERIEPS PYKFTFNKKE TCKLVCTKTY HTEKAEDKQK LEFLKKSMLL NYQHHWIVDN MPVTWCYDVE DGQRFCNPGF PIGCYITDKG HAKDACVISS DFHERDTFYI FNHVDIKIYY HVVETGSMGA RLVAAKLEPK SFKHTHIDKP DCSGPPMDIS NKASGEIKIA YTYSVSFEED DKIRWASRWD YILESMPHTH IQWFSIMNSL VIVLFLSGMV AMIMLRTLHK DIARYNQMDS TEDAQEEFGW KLVHGDIFRP PRKGMLLSVF LGSGTQILIM TFVTLFFACL GFLSPANRGA LMTCAVVLWV LLGTPAGYVA ARFYKSFGGE KWKTNVLLTS FLCPGIVFAD FFIMNLILWG EGSSAAIPFG TLVAILALWF CISVPLTFIG AYFGFKKNAI EHPVRTNQIP RQIPEQSFYT KPLPGIIMGG ILPFGCIFIQ LFFILNSIWS HQMYYMFGFL FLVFIILVIT CSEATILLCY FHLCAEDYHW QWRSFLTSGF TAVYFLIYAV HYFFSKLQIT GTASTILYFG YTMIMVLIFF LFTGTIGFFA CFWFVTKIYS VVKVD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human TM9SF2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin-free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product TM9SF2 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- TM9SF2 (Transmembrane 9 Superfamily Member 2 (TM9SF2))
- Alternative Name
- TM9SF2 (TM9SF2 Products)
- Synonyms
- P76 Protein, 1500001N15Rik Protein, AA536814 Protein, D14Ertd64e Protein, wu:fi20b05 Protein, wu:fi43d12 Protein, TM9SF2 Protein, transmembrane 9 superfamily member 2 Protein, transmembrane 9 superfamily member 2 S homeolog Protein, Transmembrane 9 superfamily member 2 Protein, TM9SF2 Protein, Tm9sf2 Protein, tm9sf2 Protein, tm9sf2.S Protein, tm9s2 Protein
- Background
- In the intracellular compartments, may function as a channel or small molecule transporter.
- Molecular Weight
- 73.9 kDa Including tag.
- UniProt
- Q99805
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