Ribophorin II Protein (RPN2) (AA 23-631) (rho-1D4 tag)
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- Target See all Ribophorin II (RPN2) Proteins
- Ribophorin II (RPN2)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-631
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This Ribophorin II protein is labelled with rho-1D4 tag.
- Application
- Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
- Sequence
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LTPTHYLTKH DVERLKASLD RPFTNLESAF YSIVGLSSLG AQVPDAKKAC TYIRSNLDPS NVDSLFYAAQ ASQALSGCEI SISNETKDLL LAAVSEDSSV TQIYHAVAAL SGFGLPLASQ EALSALTARL SKEETVLATV QALQTASHLS QQADLRSIVE EIEDLVARLD ELGGVYLQFE EGLETTALFV AATYKLMDHV GTEPSIKEDQ VIQLMNAIFS KKNFESLSEA FSVASAAAVL SHNRYHVPVV VVPEGSASDT HEQAILRLQV TNVLSQPLTQ ATVKLEHAKS VASRATVLQK TSFTPVGDVF ELNFMNVKFS SGYYDFLVEV EGDNRYIANT VELRVKISTE VGITNVDLST VDKDQSIAPK TTRVTYPAKA KGTFIADSHQ NFALFFQLVD VNTGAELTPH QTFVRLHNQK TGQEVVFVAE PDNKNVYKFE LDTSERKIEF DSASGTYTLY LIIGDATLKN PILWNVADVV IKFPEEEAPS TVLSQNLFTP KQEIQHLFRE PEKRPPTVVS NTFTALILSP LLLLFALWIR IGANVSNFTF APSTIIFHLG HAAMLGLMYV YWTQLNMFQT LKYLAILGSV TFLAGNRMLA QQAVKRTAH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human RPN2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin-free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product RPN2 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- Ribophorin II (RPN2)
- Alternative Name
- RPN2 (RPN2 Products)
- Synonyms
- RIBIIR Protein, RPN-II Protein, RPNII Protein, SWP1 Protein, RPN2 Protein, 1300012C06Rik Protein, AV261018 Protein, Rpn-2 Protein, fb95d11 Protein, fj62b10 Protein, wu:fb61f08 Protein, wu:fb74e07 Protein, wu:fb95d11 Protein, wu:fj41f11 Protein, wu:fj62b10 Protein, rpn2 Protein, ribophorin II Protein, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 Protein, ribophorin II S homeolog Protein, RPN2 Protein, Rpn2 Protein, rpn2 Protein, LOC5579328 Protein, Bm1_30625 Protein, rpn2.S Protein
- Background
- Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
- Molecular Weight
- 68.2 kDa Including tag.
- UniProt
- P04844
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