PPAPDC3 Protein (AA 1-271) (Strep Tag)
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- Target See all PPAPDC3 Proteins
- PPAPDC3 (Phosphatidic Acid Phosphatase Type 2 Domain Containing 3 (PPAPDC3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-271
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Origin
- Human
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This PPAPDC3 protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
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MPASQSRARA RDRNNVLNRA EFLSLNQPPK GGPEPRSSGR KASGPSAQPP PAGDGARERR QSQQLPEEDC MQLNPSFKGI AFNSLLAIDI CMSKRLGVCA GRAASWASAR SMVKLIGITG HGIPWIGGTI LCLVKSSTLA GQEVLMNLLL ALLLDIMTVA GVQKLIKRRG PYETSPSLLD YLTMDIYAFP AGHASRAAMV SKFFLSHLVL AVPLRVLLVL WALCVGLSRV MIGRHHVTDV LSGFVIGYLQ FRLVELVWMP SSTCQMLISA W
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product PPAPDC3 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- PPAPDC3 (Phosphatidic Acid Phosphatase Type 2 Domain Containing 3 (PPAPDC3))
- Alternative Name
- PLPP7 (PPAPDC3 Products)
- Synonyms
- MGC147268 Protein, im:7142624 Protein, zgc:77336 Protein, PPAPDC3 Protein, DKFZp468F176 Protein, C9orf67 Protein, NET39 Protein, D830019K17Rik Protein, RGD1305821 Protein, phospholipid phosphatase 7 (inactive) Protein, phospholipid phosphatase 7 Protein, plpp7 Protein, PLPP7 Protein, Plpp7 Protein
- Background
- Inactive phospholipid phosphatase 7 (Phosphatidic acid phosphatase type 2 domain-containing protein 3),FUNCTION: Plays a role as negative regulator of myoblast differentiation, in part through effects on MTOR signaling. Has no detectable enzymatic activity (By similarity). {ECO:0000250}.
- Molecular Weight
- 29.4 kDa
- UniProt
- Q8NBV4
- Pathways
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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