ZNF592 Protein (AA 1-1267) (Strep Tag)
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- Target See all ZNF592 Proteins
- ZNF592 (Zinc Finger Protein 592 (ZNF592))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1267
- Origin
- Human
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This ZNF592 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MGDMKTPDFD DLLAAFDIPD PTSLDAKEAI QTPSEENESP LKPPGICMDE SVSLSHSGSA PDVPAVSVIV KNTSRQESFE AEKDHITPSL LHNGFRGSDL PPDPHNCGKF DSTFMNGDSA RSFPGKLEPP KSEPLPTFNQ FSPISSPEPE DPIKDNGFGI KPKHSDSYFP PPLGCGAVGG PVLEALAKFP VPELHMFDHF CKKEPKPEPL PLGSQQEHEQ SGQNTVEPHK DPDATRFFGE ALEFNSHPSN SIGESKGLAR ELGTCSSVPP RQRLKPAHSK LSSCVAALVA LQAKRVASVT KEDQPGHTKD LSGPTKESSK GSPKMPKSPK SPRSPLEATR KSIKPSDSPR SICSDSSSKG SPSVAASSPP AIPKVRIKTI KTSSGEIKRT VTRILPDPDD PSKSPVGSPL GSAIAEAPSE MPGDEVPVEE HFPEAGTNSG SPQGARKGDE SMTKASDSSS PSCSSGPRVP KGAAPGSQTG KKQQSTALQA STLAPANLLP KAVHLANLNL VPHSVAASVT AKSSVQRRSQ PQLTQMSVPL VHQVKKAAPL IVEVFNKVLH SSNPVPLYAP NLSPPADSRI HVPASGYCCL ECGDAFALEK SLSQHYGRRS VHIEVLCTLC SKTLLFFNKC SLLRHARDHK SKGLVMQCSQ LLVKPISADQ MFVSAPVNST APAAPAPSSS PKHGLTSGSA SPPPPALPLY PDPVRLIRYS IKCLECHKQM RDYMVLAAHF QRTTEETEGL TCQVCQMLLP NQCSFCAHQR IHAHKSPYCC PECGVLCRSA YFQTHVKENC LHYARKVGYR CIHCGVVHLT LALLKSHIQE RHCQVFHKCA FCPMAFKTAS STADHSATQH PTQPHRPSQL IYKCSCEMVF NKKRHIQQHF YQNVSKTQVG VFKCPECPLL FVQKPELMQH VKSTHGVPRN VDELSSLQSS ADTSSSRPGS RVPTEPPATS VAARSSSLPS GRWGRPEAHR RVEARPRLRN TGWTCQECQE WVPDRESYVS HMKKSHGRTL KRYPCRQCEQ SFHTPNSLRK HIRNNHDTVK KFYTCGYCTE DSPSFPRPSL LESHISLMHG IRNPDLSQTS KVKPPGGHSP QVNHLKRPVS GVGDAPGTSN GATVSSTKRH KSLFQCAKCS FATDSGLEFQ SHIPQHQVDS STAQCLLCGL CYTSASSLSR HLFIVHKVRD QEEEEEEEAA AAEMAVEVAE PEEGSGEEVP METRENGLEE CAGEPLSADP EARRLLGPAP EDDGGHNDHS QPQASQDQDS HTLSPQV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product ZNF592 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ZNF592 (Zinc Finger Protein 592 (ZNF592))
- Alternative Name
- ZNF592 (ZNF592 Products)
- Synonyms
- Znf592 Protein, MGC68936 Protein, MGC145098 Protein, MGC159636 Protein, wu:fj94a04 Protein, CAMOS Protein, SCAR5 Protein, 8430405N24 Protein, A730014M16Rik Protein, mKIAA0211 Protein, zinc finger protein 592 Protein, zinc finger protein 592 L homeolog Protein, Zfp592 Protein, znf592.L Protein, ZNF592 Protein, znf592 Protein
- Background
- Zinc finger protein 592,FUNCTION: May be involved in transcriptional regulation. {ECO:0000269|PubMed:20531441}.
- Molecular Weight
- 137.5 kDa
- UniProt
- Q92610
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