ZNF532 Protein (AA 1-1301) (Strep Tag)
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- Target See all ZNF532 products
- ZNF532 (Zinc Finger Protein 532 (ZNF532))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1301
- Origin
- Human
- Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This ZNF532 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MTMGDMKTPD FDDLLAAFDI PDMVDPKAAI ESGHDDHESH MKQNAHGEDD SHAPSSSDVG VSVIVKNVRN IDSSEGGEKD GHNPTGNGLH NGFLTASSLD SYSKDGAKSL KGDVPASEVT LKDSTFSQFS PISSAEEFDD DEKIEVDDPP DKEDMRSSFR SNVLTGSAPQ QDYDKLKALG GENSSKTGLS TSGNVEKNKA VKRETEASSI NLSVYEPFKV RKAEDKLKES SDKVLENRVL DGKLSSEKND TSLPSVAPSK TKSSSKLSSC IAAIAALSAK KAASDSCKEP VANSRESSPL PKEVNDSPRA ADKSPESQNL IDGTKKPSLK QPDSPRSISS ENSSKGSPSS PAGSTPAIPK VRIKTIKTSS GEIKRTVTRV LPEVDLDSGK KPSEQTASVM ASVTSLLSSP ASAAVLSSPP RAPLQSAVVT NAVSPAELTP KQVTIKPVAT AFLPVSAVKT AGSQVINLKL ANNTTVKATV ISAASVQSAS SAIIKAANAI QQQTVVVPAS SLANAKLVPK TVHLANLNLL PQGAQATSEL RQVLTKPQQQ IKQAIINAAA SQPPKKVSRV QVVSSLQSSV VEAFNKVLSS VNPVPVYIPN LSPPANAGIT LPTRGYKCLE CGDSFALEKS LTQHYDRRSV RIEVTCNHCT KNLVFYNKCS LLSHARGHKE KGVVMQCSHL ILKPVPADQM IVSPSSNTST STSTLQSPVG AGTHTVTKIQ SGITGTVISA PSSTPITPAM PLDEDPSKLC RHSLKCLECN EVFQDETSLA THFQQAADTS GQKTCTICQM LLPNQCSYAS HQRIHQHKSP YTCPECGAIC RSVHFQTHVT KNCLHYTRRV GFRCVHCNVV YSDVAALKSH IQGSHCEVFY KCPICPMAFK SAPSTHSHAY TQHPGIKIGE PKIIYKCSMC DTVFTLQTLL YRHFDQHIEN QKVSVFKCPD CSLLYAQKQL MMDHIKSMHG TLKSIEGPPN LGINLPLSIK PATQNSANQN KEDTKSMNGK EKLEKKSPSP VKKSMETKKV ASPGWTCWEC DCLFMQRDVY ISHVRKEHGK QMKKHPCRQC DKSFSSSHSL CRHNRIKHKG IRKVYACSHC PDSRRTFTKR LMLEKHVQLM HGIKDPDLKE MTDATNEEET EIKEDTKVPS PKRKLEEPVL EFRPPRGAIT QPLKKLKINV FKVHKCAVCG FTTENLLQFH EHIPQHKSDG SSYQCRECGL CYTSHVSLSR HLFIVHKLKE PQPVSKQNGA GEDNQQENKP SHEDESPDGA VSDRKCKVCA KTFETEAALN THMRTHGMAF IKSKRMSSAE K
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ZNF532 (Zinc Finger Protein 532 (ZNF532))
- Alternative Name
- ZNF532 (ZNF532 Products)
- Synonyms
- C530030I18Rik Protein, Znf532 Protein, mKIAA1629 Protein, zinc finger protein 532 Protein, ZNF532 Protein, Zfp532 Protein
- Background
- Zinc finger protein 532,FUNCTION: May be involved in transcriptional regulation.
- Molecular Weight
- 141.7 kDa
- UniProt
- Q9HCE3
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