SSFA2 Protein (AA 1-1259) (His tag)
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- Target See all SSFA2 Proteins
- SSFA2 (Sperm Specific Antigen 2 (SSFA2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1259
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This SSFA2 protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequence
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MDRPLSSSAE AEEELEWQVA SRRRKAWAKC RSSWQASETE DLSTEATTQD EEEDEEEDLP GAQLPAAGGR GNVPNEKIAI WLKDCRTPLG ASLDEQSSST LKGVLVRNGG SFEDDLSLGA EANHLHESDA QIENCNNILA KERRLQFHQK GRSMNSTGSG KSSGTVSSVS ELLELYEEDP EEILYNLGFG RDEPDIASKI PSRFFNSSSF AKGIDIKVFL SAQMQRMEVE NPNYALTSRF RQIEVLTTVA NAFSSLYSQV SGTPLQRIGS MSSVTSNKET DPPPPLTRSN TANRLMKTLS KLNLCVDKTE KGESSSPSPS AEKGKILNVS VIEESGNKND QKSQKIMKKK ESSSMLATVK EEVSGSSAAV TENADSDRIS DEANSNFNQG TENEQSKETQ SHESKLGEES GIVESKLDSD FNISSHSELE NSSELKSVHI STPEKEPCAP LTIPSIRNIM TQQKDSFEME EVQSTEGEAP HVPATYQLGL TKSKRDHLLR TASQHSDSSG FAEDSTDCLS LNHLQVQESL QAMGSSADSC DSETTVTSLG EDLATPTAQD QPYFNESEEE SLVPLQKGLE KAAAVADKRK SGSQDFPQCN TIENTGTKQS TCSPGDHIIE ITEVEEDLFP AETVELLREA SAESDVGKSS ESEFTQYTTH HILKSLASIE AKCSDMSSEN TTGPPSSMDR VNTALQRAQM KVCSLSNQRM GRSLLKSKDL LKQRYLFAKA GYPLRRSQSL PTTLLSPVRV VSSVNVRLSP GKETRCSPPS FTYKYTPEEE QELEKRVMEH DGQSLVKSTI FISPSSVKKE EAPQSEAPRV EECHHGRTPT CSRLAPPPMS QSTCSLHSIH SEWQERPLCE HTRTLSTHSV PNISGATCSA FASPFGCPYS HRHATYPYRV CSVNPPSAIE MQLRRVLHDI RNSLQNLSQY PMMRGPDPAA APYSTQKSSV LPLYENTFQE LQVMRRSLNL FRTQMMDLEL AMLRQQTMVY HHMTEEERFE VDQLQGLRNS VRMELQDLEL QLEERLLGLE EQLRAVRMPS PFRSSALMGM CGSRSADNLS CPSPLNVMEP VTELMQEQSY LKSELGLGLG EMGFEIPPGE SSESVFSQAT SESSSVCSGP SHANRRTGVP STASVGKSKT PLVARKKVFR ASVALTPTAP SRTGSVQTPP DLESSEEVDA AEGAPEVVGP KSEVEEGHGK LPSMPAAEEM HKNVEQDELQ QVIREIKESI VGEIRREIVS GLLAAVSSSK ASNSKQDYH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human SSFA2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product SSFA2 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- SSFA2 (Sperm Specific Antigen 2 (SSFA2))
- Alternative Name
- SSFA2 (SSFA2 Products)
- Synonyms
- si:dkey-165l22.3 Protein, MGC146293 Protein, DKFZp469G2317 Protein, CS-1 Protein, CS1 Protein, KRAP Protein, SPAG13 Protein, 5730488C15Rik Protein, mKIAA1927 Protein, sperm specific antigen 2 Protein, zinc finger SWIM-type containing 2 Protein, sperm-specific antigen 2 Protein, SSFA2 Protein, ssfa2 Protein, Ssfa2 Protein, ZSWIM2 Protein, LOC101120052 Protein
- Molecular Weight
- 139.3 kDa Including tag.
- UniProt
- P28290
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