RAB11FIP1 Protein (AA 1-1283) (Strep Tag)
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- Target See all RAB11FIP1 Proteins
- RAB11FIP1 (Rab11 Family-Interacting Protein 1 (RAB11FIP1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1283
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Origin
- Human
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This RAB11FIP1 protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
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MSLMVSAGRG LGAVWSPTHV QVTVLQARGL RAKGPGGTSD AYAVIQVGKE KYATSVSERS LGAPVWREEA TFELPSLLSS GPAAAATLQL TVLHRALLGL DKFLGRAEVD LRDLHRDQGR RKTQWYKLKS KPGKKDKERG EIEVDIQFMR NNMTASMFDL SMKDKSRNPF GKLKDKIKGK NKDSGSDTAS AIIPSTTPSV DSDDESVVKD KKKKSKIKTL LSKSNLQKTP LSQSMSVLPT SKPEKVLLRP GDFQSQWDED DNEDESSSAS DVMSHKRTAS TDLKQLNQVN FTLPKKEGLS FLGGLRSKND VLSRSNVCIN GNHVYLEQPE AKGEIKDSSP SSSPSPKGFR KKHLFSSTEN LAAGSWKEPA EGGGLSSDRQ LSESSTKDSL KSMTLPSYRP APLVSGDLRE NMAPANSEAT KEAKESKKPE SRRSSLLSLM TGKKDVAKGS EGENPLTVPG REKEGMLMGV KPGEDASGPA EDLVRRSEKD TAAVVSRQGS SLNLFEDVQI TEPEAEPESK SEPRPPISSP RAPQTRAVKP RLEVSPEAQP TARLPSPTDS PSSLPPLPSS SGQASVPSEL GHGADTQSSE SPSVFSSLSS PIAAPISTST PIESWPLVDR GQAKSEGPPL LPKAELQTES LTPVPNSGSS ALGSLFKQPS FPANKGTEDS LMGRTRETGT EKNTSSLELE ESLPEQPETG RQEEELPRFP CKKQDYSPSS GEAQEVPFAL SLSSDGAVSP VGELAAGGDR DLESQAGSLV ESKARDAAEE VAPPLPMGAS VPSIDSMMRK LEEMGLNLRK DQKKTKKRVS FSEQLFTEEA VAGAALLVEG HSSCPQELNP AWSVAGNASD GEPPESPHAE DSERESVTTP GPATCGAPAS PADHLLLPSQ EESFSEVPMS EASSAKDTPL FRMEGEDALV TQYQSKASDH EGLLSDPLSD LQLVSDFKSP IMADLNLSLP SIPEVASDDE RIDQVEDDGD QVEDDGETAK SSTLDIGALS LGLVVPCPER GKGPSGEADR LVLGEGLCDF RLQAPQASVT APSEQTTEFG IHKPHLGKSS SLDKQLPGPS GGEEEKPMGN GSPSPPPGTS LDNPVPSPSP SEIFPVTHSF PSSAHSDTHH TSTAESQKKA TAEGSAGRVE NFGKRKPLLQ AWVSPSETHP VSAQPGAGTG SAKHRLHPVK PMNAMATKVA NCSLGTATII SENLNNEVMM KKYSPSDPAF AYAQLTHDEL IQLVLKQKET ISKKEFQVRE LEDYIDNLLV RVMEETPNIL RIPTQVGKKA GKM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product RAB11FIP1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- RAB11FIP1 (Rab11 Family-Interacting Protein 1 (RAB11FIP1))
- Alternative Name
- RAB11FIP1 (RAB11FIP1 Products)
- Synonyms
- zgc:122989 Protein, NOEL1A Protein, RCP Protein, rab11-FIP1 Protein, 2010200K21Rik Protein, 4833414G05Rik Protein, Rcp Protein, RGD1562965 Protein, Rab11-FIP1 Protein, RAB11 family interacting protein 1 Protein, RAB11 family interacting protein 1 (class I) a Protein, RAB11 family interacting protein 1 (class I) Protein, RAB11FIP1 Protein, rab11fip1a Protein, Rab11fip1 Protein
- Background
- Rab11 family-interacting protein 1 (Rab11-FIP1) (Rab-coupling protein),FUNCTION: A Rab11 effector protein involved in the endosomal recycling process. Also involved in controlling membrane trafficking along the phagocytic pathway and in phagocytosis. {ECO:0000269|PubMed:11786538, ECO:0000269|PubMed:15181150, ECO:0000269|PubMed:15355514, ECO:0000269|PubMed:16920206}.
- Molecular Weight
- 137.2 kDa
- UniProt
- Q6WKZ4
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