C17ORF85 Protein (AA 1-620) (Strep Tag)
-
- Target See all C17ORF85 products
- C17ORF85 (Chromosome 17 Open Reading Frame 85 (C17ORF85))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-620
-
Origin
- Human
-
Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This C17ORF85 protein is labelled with Strep Tag.
- Application
- Western Blotting (WB), ELISA, SDS-PAGE (SDS)
- Sequence
-
MAAVRGLRVS VKAEAPAGPA LGLPSPEAES GVDRGEPEPM EVEEGELEIV PVRRSLKELI PDTSRRYENK AGSFITGIDV TSKEAIEKKE QRAKRFHFRS EVNLAQRNVA LDRDMMKKAI PKVRLETIYI CGVDEMSTQD VFSYFKEYPP AHIEWLDDTS CNVVWLDEMT ATRALINMSS LPAQDKIRSR DASEDKSAEK RKKDKQEDSS DDDEAEEGEV EDENSSDVEL DTLSQVEEES LLRNDLRPAN KLAKGNRLFM RFATKDDKKE LGAARRSQYY MKYGNPNYGG MKGILSNSWK RRYHSRRIQR DVIKKRALIG DDVGLTSYKH RHSGLVNVPE EPIEEEEEEE EEEEEEEEED QDMDADDRVV VEYHEELPAL KQPRERSASR RSSASSSDSD EMDYDLELKM ISTPSPKKSM KMTMYADEVE SQLKNIRNSM RADSVSSSNI KNRIGNKLPP EKFADVRHLL DEKRQHSRPR PPVSSTKSDI RQRLGKRPHS PEKAFSSNPV VRREPSSDVH SRLGVPRQDS KGLYADTREK KSGNLWTRLG SAPKTKEKNT KKVDHRAPGA EEDDSELQRA WGALIKEKEQ SRQKKSRLDN LPSLQIEVSR ESSSGSEAES
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Endotoxin Level
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
-
-
- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
-
- Target
- C17ORF85 (Chromosome 17 Open Reading Frame 85 (C17ORF85))
- Alternative Name
- NCBP3 (C17ORF85 Products)
- Synonyms
- ELG Protein, HSA277841 Protein, C130061O14Rik Protein, C78393 Protein, nuclear cap binding subunit 3 Protein, nuclear cap binding subunit 3 L homeolog Protein, NCBP3 Protein, Ncbp3 Protein, ncbp3.L Protein
- Background
- Nuclear cap-binding protein subunit 3 (Protein ELG),FUNCTION: Associates with NCBP1/CBP80 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export. NCBP3 serves as adapter protein linking the capped RNAs (m7GpppG-capped RNA) to NCBP1/CBP80. Unlike the conventional CBC with NCBP2 which binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus, the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role in only mRNA export. The alternative CBC is particularly important in cellular stress situations such as virus infections and the NCBP3 activity is critical to inhibit virus growth (PubMed:26382858). {ECO:0000269|PubMed:26382858}.
- Molecular Weight
- 70.6 kDa
- UniProt
- Q53F19
-