MAGEE1 Protein (AA 1-957) (Strep Tag)
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- Target See all MAGEE1 products
- MAGEE1 (Melanoma Antigen Family E, 1 (MAGEE1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-957
- Origin
- Human
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This MAGEE1 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MSLVSQNSRR RRRRVAKATA HNSSWGEMQA PNAPGLPADV PGSDVPQGPS DSQILQGLCA SEGPSTSVLP TSAEGPSTFV PPTISEASSA SGQPTISEGP GTSVLPTPSE GLSTSGPPTI SKGLCTSVTL AASEGRNTSR PPTSSEEPST SVPPTASEVP STSLPPTPGE GTSTSVPPTA YEGPSTSVVP TPDEGPSTSV LPTPGEGPGT SVPLAATEGL STSVQATPDE GPSTSVPPTA TEGLSTPVPP TRDEGPSTSV PATPGEGPST SVLPAASDGQ SISLVPTRGK GSSTSVPPTA TEGLSTSVQP TAGEGSSTSV PPTPGGGLST SVPPTATEEL STSVPPTPGE GPSTSVLPIP GEGLSTSVPP TASDGSDTSV PPTPGEGAST LVQPTAPDGP GSSVLPNPGE GPSTLFSSSA SVDRNPSKCS LVLPSPRVTK ASVDSDSEGP KGAEGPIEFE VLRDCESPNS ISIMGLNTSR VAITLKPQDP MEQNVAELLQ FLLVKDQSKY PIRESEMREY IVKEYRNQFP EILRRAAAHL ECIFRFELRE LDPEAHTYIL LNKLGPVPFE GLEESPNGPK MGLLMMILGQ IFLNGNQAKE AEIWEMLWRM GVQRERRLSI FGNPKRLLSV EFVWQRYLDY RPVTDCKPVE YEFFWGPRSH LETTKMKILK FMAKIYNKDP MDWPEKYNEA LEEDAARAFA EGWQALPHFR RPFFEEAAAE VPSPDSEVSS YSSKYAPHSW PESRLESKAR KLVQLFLLMD STKLPIPKKG ILYYIGRECS KVFPDLLNRA ARTLNHVYGT ELVVLDPRNH SYTLYNRREM EETEEIVDSP NRPGNNFLMQ VLSFIFIMGN HARESAVWAF LRGLGVQAGR KHVITCRYLS QRYIDSLRVP DSDPVQYEFV WGPRARLETS KMKALRYVAR IHRKEPQDWP QQYREAMEDE ANRADVGHRQ IFVHNFR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- MAGEE1 (Melanoma Antigen Family E, 1 (MAGEE1))
- Alternative Name
- MAGEE1 (MAGEE1 Products)
- Synonyms
- hca4 Protein, magee1 Protein, mageg1 Protein, magel3 Protein, nse3 Protein, nsmce3 Protein, DAMAGE Protein, HCA1 Protein, AI847422 Protein, mMage-e1 Protein, RGD1560259 Protein, NSE3 homolog, SMC5-SMC6 complex component Protein, MAGE family member E1 Protein, melanoma antigen, family E, 1 Protein, nsmce3 Protein, MAGEE1 Protein, Magee1 Protein
- Background
- Melanoma-associated antigen E1 (Alpha-dystrobrevin-associated MAGE Protein) (DAMAGE) (Hepatocellular carcinoma-associated protein 1) (MAGE-E1 antigen),FUNCTION: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. {ECO:0000269|PubMed:20864041}.
- Molecular Weight
- 103.3 kDa
- UniProt
- Q9HCI5
- Pathways
- Synaptic Membrane
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