C1ORF74 Protein (AA 1-269) (Strep Tag)
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- Target See all C1ORF74 Proteins
- C1ORF74 (Chromosome 1 Open Reading Frame 74 (C1ORF74))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-269
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Origin
- Human
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This C1ORF74 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MLLLDLMSSP SPQLLVAAAQ QTLGMGKRRS PPQAICLHLA GEVLAVARGL KPAVLYDCNC AGASELQSYL EELKGLGFLT FGLHILEIGE NSLIVSPEHV CQHLEQVLLG TIAFVDVSSC QRHPSVCSLD QLQDLKALVA EIITHLQGLQ RDLSLAVSYS RLHSSDWNLC TVFGILLGYP VPYTFHLNQG DDNCLALTPL RVFTARISWL LGQPPILLYS FSVPESLFPG LRDILNTWEK DLRTRFRTQN DFADLSISSE IVTLPAVAL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- C1ORF74 (Chromosome 1 Open Reading Frame 74 (C1ORF74))
- Alternative Name
- C1orf74 (C1ORF74 Products)
- Synonyms
- C1orf74 Protein, DKFZp469F2128 Protein, chromosome 1 open reading frame 74 Protein, chromosome 1 open reading frame 74 L homeolog Protein, chromosome 26 C1orf74 homolog Protein, hypothetical protein LOC100125367 Protein, chromosome 1 open reading frame, human C1orf74 Protein, RIKEN cDNA A130010J15 gene Protein, chromosome ssa22 open reading frame, human C1orf74 Protein, chromosome 16 open reading frame, human C1orf74 Protein, zgc:112163 Protein, C1orf74 Protein, c1orf74.L Protein, c1orf74 Protein, C26H1orf74 Protein, LOC100125367 Protein, C1H1orf74 Protein, A130010J15Rik Protein, cssa22h1orf74 Protein, C16H1orf74 Protein, zgc:112163 Protein
- Background
- UPF0739 protein C1orf74
- Molecular Weight
- 29.6 kDa
- UniProt
- Q96LT6
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