Protein A Protein (Biotin)

Catalog No. ABIN3041808

Product Details

Target: Protein A

Protein Type: Native

Origin: Staphylococcus aureus

Source: Staphylococcus aureus

Purification tag / Conjugate: This Protein A protein is labelled with Biotin.

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Characteristics: Protein A is the component of the cytoderm of staphylococcus aureus. It was regarded as a kind of broad spectrum secondary antibody, because it can bind Fc fragments of a majority of immunoglobulin. It can react with guinea pig, rabbit globulin and human IgG1, 2, 4 strongly and react with mouse, bovine globulin and human IgA, E, M weakly.

Application Details

Application Notes: This protein must be used with various kinds of enzymes or fluorescence conjugated avidin (streptavidin) in IHC, ICC, WB and ELISA.

Western blot(DAB)|2.5-5 μg/mL| Western blot(ECM)|0.1-0.3 μg/mL| Immunohistochemistry(Paraffin-embedded Section)|5-10 μg/mL| Immunohistochemistry(Frozen Section)|5-10 μg/mL| Immunocytochemistry|5-10 μg/mL| ELISA|0.05-0.2 μg/mL|

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Storage: 4 °C

Storage Comment: At 4°C for one year.

Expiry Date: 12 months

References

Liu, Liu, Chen, Maitz, Chen, Huang: "Influence of a layer-by-layer-assembled multilayer of anti-CD34 antibody, vascular endothelial growth factor, and heparin on the endothelialization and anticoagulation of titanium surface." in: Journal of biomedical materials research. Part A, Vol. 101, Issue 4, pp. 1144-57, (2013) (PubMed).

Li, Huang, Chen, Chen, Xiong, Chen, You, Jin, Liang: "Oriented immobilization of anti-CD34 antibody on titanium surface for self-endothelialization induction." in: Journal of biomedical materials research. Part A, Vol. 94, Issue 4, pp. 1283-93, (2010) (PubMed).

Target Details

Target: Protein A

Abstract: Protein A Products

Background: Protein A is the component of the cytoderm of staphylococcus aureus. It was regarded as a kind of broad spectrum secondary antibody, because it can bind Fc fragments of a majority of immunoglobulin. It can react with guinea pig, rabbit globulin and human IgG1, 2, 4 strongly and react with mouse, bovine globulin and human IgA, E, M weakly.