Streptavidin Protein (HRP)

Catalog No. ABIN3041800

Product Details

Target: Streptavidin

Protein Type: Native

Origin: Streptomyces avidinii

Source: Streptomyces avidinii

Purification tag / Conjugate: This Streptavidin protein is labelled with HRP.

Application: Western Blotting (WB), ELISA, Dot Blot (DB)

Characteristics: Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-HRP can form a complex which composed by about one hundred HRP and fifty streptavidin. And lots of enzymes can ensure the streptavidin-HRP with high sensitivity.

Application Details

Application Notes: Dot blot(ECM)|0.25-0.5 μg/mL| Western blot(DAB)|0.3-2 μg/mL| Western blot(ECM)|0.1-0.3 μg/mL| ELISA|0.025-0.05 μg/mL|

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Storage: 4 °C

Storage Comment: At 4°C for one year.

Expiry Date: 12 months

References

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Target Details

Target: Streptavidin

Abstract: Streptavidin Products

Background: Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-HRP can form a complex which composed by about one hundred HRP and fifty streptavidin. And lots of enzymes can ensure the streptavidin-HRP with high sensitivity.