DKK3 Protein (AA 23-349) (His tag)

Catalog No. ABIN2870735

Product Details

Target: DKK3

Protein Type: Recombinant

Protein Characteristics: AA 23-349

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This DKK3 protein is labelled with His tag.

Sequence: AA 23-349

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.1 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: DKK3

Alternative Name: Dkk-3 (DKK3 Products)

Background: Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumour suppressor gene and a marker for tumour blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.

Molecular Weight: 37.1 kDa

NCBI Accession: NP_056629