FBXW7 Protein (AA 1-553) (GST tag)

Catalog No. ABIN1990130

Product Details

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Protein Type: Recombinant

Protein Characteristics: AA 1-553

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This FBXW7 protein is labelled with GST tag.

Application: ELISA, Western Blotting (WB), Antibody Array (AA), Affinity Purification (AP)

Purpose: FBXW7 (Human) Recombinant Protein (P01)

Sequence: MGFYGTLKMI FYKMKRKLDH GSEVRSFSLG KKPCKVSEYT STTGLVPCSA TPTTFGDLRA ANGQGQQRRR ITSVQPPTGL QEWLKMFQSW SGPEKLLALD ELIDSCEPTQ VKHMMQVIEP QFQRDFISLL PKELALYVLS FLEPKDLLQA AQTCRYWRIL AEDNLLWREK CKEEGIDEPL HIKRRKVIKP GFIHSPWKSA YIRQHRIDTN WRRGELKSPK VLKGHDDHVI TCLQFCGNRI VSGSDDNTLK VWSAVTGKCL RTLVGHTGGV WSSQMRDNII ISGSTDRTLK VWNAETGECI HTLYGHTSTV RCMHLHEKRV VSGSRDATLR VWDIETGQCL HVLMGHVAAV RCVQYDGRRV VSGAYDFMVK VWDPETETCL HTLQGHTNRV YSLQFDGIHV VSGSLDTSIR VWDVETGNCI HTLTGHQSLT SGMELKDNIL VSGNADSTVK IWDIKTGQCL QTLQGPNKHQ SAVTCLQFNK NFVITSSDDG TVKLWDLKTG EFIRNLVTLE SGGSGGVVWR IRASNTKLVC AVGSRNGTEE TKLLVLDFDV DMK

Characteristics: Human FBXW7 full-length ORF (BAA91986.1, 1 a.a. - 553 a.a.) recombinant protein with GST tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

Target Details

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Alternative Name: FBXW7 (FBXW7 Products)

Synonyms: cdc4 Protein, AGO Protein, CDC4 Protein, FBW6 Protein, FBW7 Protein, FBX30 Protein, FBXO30 Protein, FBXW6 Protein, SEL-10 Protein, SEL10 Protein, hAgo Protein, hCdc4 Protein, 1110001A17Rik Protein, Cdc4 Protein, Fbw7 Protein, Fbwd6 Protein, Fbx30 Protein, Fbxo30 Protein, Fbxw6 Protein, F-box and WD repeat domain containing 7 Protein, F-box and WD-40 domain protein 7 Protein, Fbxw7 Protein, FBXW7 Protein, fbxw7 Protein

Background: Synonyms: AGO,CDC4,DKFZp686F23254,FBW6,FBW7,FBX30,FBXO30,FBXW6,FLJ16457,SEL-10,SEL10
Gene Description: F-box and WD repeat domain containing 7
Gene Name: FBXW7
Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class, in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Three transcript variants encoding three different isoforms have been found for this gene.
GenBank: AK001933.1, BAA91986.1

Molecular Weight: 88.7 kDa (theoreatical)

Gene ID: 55294

Pathways: Notch Signaling, EGFR Signaling Pathway