VEGF 165 (Active) Protein
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- Target
- VEGF 165
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Cross-Reactivity
- Human
- Characteristics
- Human VEGF 165 (165aa).
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.1ng/μg protein.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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The ED50 for stimulation of cell proliferation in human umbilical vein endothelial cells by VEGF 165 has been determined to be in the range of 1-5ng/ml.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with 50 mM acetic acid to a concentration not lower than 50 μg/mL.
- Concentration
- Lot specific
- Buffer
- Lyophilized.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - Expiry Date
- 6 months
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- Target
- VEGF 165
- Background
- Human vascular endothelial growth factor (VEGF 165) is produced as a homodimer. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. VEGF 165 is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. It is also a chemoattractant molecule for monocytes and endothelial cells. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels.
- Molecular Weight
- ~38.2kDa
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