NADK Protein
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- Target See all NADK Proteins
- NADK (NAD Kinase (NADK))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Bacillus subtilis
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Characteristics
- Full-length NAD kinase (Bacillus subtilis).
- Purity
- >98 % (SDS-PAGE)
- Top Product
- Discover our top product NADK Protein
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NAD Kinase (NADK) (AA 64-298) protein (His tag)
NADK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
NAD Kinase (NADK) (AA 1-446) protein (GST tag)NADK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
NAD Kinase (NADK) (AA 1-110) protein (GST tag)NADK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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~2.6 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol nicotinamide adenine dinucleotide phosphate (NADP) per min.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. In 50 mM TRIS-HCl, pH 7.0 containing 150 mM sodium chloride, 1 mM EDTA and 1 mM dithiothreitol.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
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Short Term Storage: -20°C
Long Term Storage: -80°C
Stable for at least 6 months after receipt when stored at -80°C. - Expiry Date
- 6 months
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- Target
- NADK (NAD Kinase (NADK))
- Alternative Name
- NAD Kinase (NADK Products)
- Background
- NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Bacillus subtilis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Catalytic activity: ATP + NAD+ = ADP + NADP+
- Molecular Weight
- ~30.0kDa
- UniProt
- O31612
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